Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

Anti-FLI1 Picoband® Antibody

Boster Bio Anti-FLI1 Picoband® Antibody catalog # A00399. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Friend leukemia integration 1 transcription factor (FLI1), also known as transcription factor ERGB, is a protein that in humans is encoded by the FLI1 gene, which is a proto-oncogene. It is mapped to 11q24.3. This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t (11;22) (q24; q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t (4;11) (q21; q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants.

Synonyms

Friend leukemia integration 1 transcription factor; Proto-oncogene Fli-1; Transcription factor ERGB; FLI1

Gene Name

FLI1

Gene ID

2313

UniProt

Q01543

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human FLI1 recombinant protein (Position: M1-Y452) .

Clonality

Polyclonal

Tissue Specificity

Expressed in most tissues tested, including skin, lung, kidney, heart, testis and stomach.

Applications

WB, IHC, ICC, IF, Flow Cytometry, ELISA

Field of Research

Class I, Cytoskeleton, Cytoskeleton/ECM, Intermediate Filaments, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.

References & Citations

1. Lin, P. P., Brody, R. I., Hamelin, A. C., Bradner, J. E., Healey, J. H., Ladanyi, M. Differential transactivation by alternative EWS-FLI1 fusion proteins correlates with clinical heterogeneity in Ewing's sarcoma. Cancer Res. 59: 1428-1432, 1999. 2. May, W. A., Gishizky, M. L., Lessnick, S. L., Lunsford, L. B., Lewis, B. C., Delattre, O., Zucman, J., Thomas, G., Denny, C. T. Ewing sarcoma 11;22 translocation produces a chimeric transcription factor that requires the DNA-binding domain encoded by FLI1 for transformation. Proc. Nat. Acad. Sci. 90: 5752-5756, 1993. 3. Nakatani, F., Tanaka, K., Sakimura, R., Matsumoto, Y., Matsunobu, T., Li, X., Hanada, M., Okada, T., Iwamoto, Y. Identification of p21 (WAF1/CIP1) as a target of EWS-Fli1 oncogenic fusion protein. J. Biol. Chem. 278: 15105-15115, 2003.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

62378 MW

Observed Molecular Weight

54 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Fli-1 proto-oncogene, ETS transcription factor

Subcellular Location

Nucleus.

Protein Name

Keratin, type II cytoskeletal 5

Isotype

Rabbit IgG

Contents

243

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

Rabbit Polyclonal HMGB3/HMG4 Antibody [DyLight 594]
NBP2-98886DL594 0.1 mL

Rabbit Polyclonal HMGB3/HMG4 Antibody [DyLight 594]

Ask
View Details
PRL (Prolactin) (Biotin)
MBS6174316-01 0.1 mL

PRL (Prolactin) (Biotin)

Ask
View Details
PRL (Prolactin) (Biotin)
MBS6174316-02 5x 0.1 mL

PRL (Prolactin) (Biotin)

Ask
View Details
RNF35 Polyclonal Antibody, AbBy Fluor™ 750 Conjugated
bs-9237R-BF750 100 µL

RNF35 Polyclonal Antibody, AbBy Fluor™ 750 Conjugated

Ask
View Details
Nilutamide discontinued. See 285151
018140 10 mg

Nilutamide discontinued. See 285151

Ask
View Details