Anti-Aryl hydrocarbon Receptor/Ahr Antibody Picoband®

AHR (aryl hydrocarbon receptor), also called bHLHe76,   is a member of the family of  basic helix-loop-helix  transcription factors.  AhR is a cytosolic transcription factor that is normally inactive, bound to several  co-chaperones. The AHR gene is mapped on 7p21.1. Estrogenic actions of  AHR  agonists were detected in wildtype ovariectomized mouse uteri, but were absent in  Ahr  -/- or Er-alpha -/- ovariectomized mice. Complex assembly and ubiquitin ligase activity of CUL4B (AHR) in vitro and in vivo are dependent on the  AHR  ligand. In the CUL4B (AHR) complex, ligand-activated  AHR  acts as a substrate-specific adaptor component that targets sex steroid receptors for degradation.  Cd4-positive cells from mice lacking  Ahr  developed Th17 responses but failed to produce Il22 and did not show enhanced Th17 development. Activation of  Ahr  during induction of EAE accelerated disease onset and increased pathology in wildtype mice, but not in  Ahr  -/- mice. The TDO-AHR  pathway is active in human brain tumors and is associated with malignant progression and poor survival.  Ahr  activity within ROR-gamma-t-positive ILC could be induced by dietary ligands such as those contained in vegetables of the family Brassicaceae.&nbsp

Ligand-activated transcriptional activator. Binds to the XRE promoter region of genes it activates. Activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene) . Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons. Involved in cell-cycle regulation. Likely to play an important role in the development and maturation of many tissues. Regulates the circadian clock by inhibiting the basal and circadian expression of the core circadian component PER1. Inhibits PER1 by repressing the CLOCK-ARNTL/BMAL1 heterodimer mediated transcriptional activation of PER1. The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription.

1. Matikainen, T., Perez, G. I., Jurisicova, A., Pru, J. K., Schlezinger, J. J., Ryu, H.-Y., Laine, J., Sakai, T., Korsmeyer, S. J., Casper, R. F., Sherr, D. H., Tilly, J. L. Aromatic hydrocarbon receptor-driven Bax gene expression is required for premature ovarian failure caused by biohazardous environmental chemicals. Nature Genet. 28: 355-360, 2001. 2. Micka, J., Milatovich, A., Menon, A., Grabowski, G. A., Puga, A., Nebert, D. W. Human Ah receptor (AHR) gene: localization to 7p15 and suggestive correlation of polymorphism with CYP1A1 inducibility. Pharmacogenetics 7: 95-101, 1997. 3. Moura-Alves, P., Fae, K., Houthuys, E., Dorhoi, A., Kreuchwig, A., Furkert, J., Barison, N., Diehl, A., Munder, A., Constant, P., Skrahina, T., Guhlich-Bornhof, U., and 14 others. AhR sensing of bacterial pigments regulates antibacterial defence. Nature 512: 387-392, 2014.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.