Anti-SLBP Antibody Picoband®

Histone RNA hairpin-binding protein is a protein that in human is encoded by the SLBP gene. This gene is mapped to 4p16.3. This gene encodes a protein that binds to the stem-loop structure in replication-dependent histone mRNAs. Histone mRNAs do not contain introns or polyadenylation signals, and are processed by endonucleolytic cleavage. The stem-loop structure is essential for efficient processing but this structure also controls the transport, translation and stability of histone mRNAs. Expression of the protein is regulated during the cell cycle, increasing more than 10-fold during the latter part of G1.

RNA-binding protein involved in the histone pre-mRNA processing. Binds the stem-loop structure of replication-dependent histone pre-mRNAs and contributes to efficient 3'-end processing by stabilizing the complex between histone pre-mRNA and U7 small nuclear ribonucleoprotein (snRNP), via the histone downstream element (HDE) . Plays an important role in targeting mature histone mRNA from the nucleus to the cytoplasm and to the translation machinery. Stabilizes mature histone mRNA and could be involved in cell-cycle regulation of histone gene expression. Involved in the mechanism by which growing oocytes accumulate histone proteins that support early embryogenesis. Binds to the 5' side of the stem-loop structure of histone pre-mRNAs.

1. Martin, F., Schaller, A., Eglite, S., Schumperli, D., Muller, B. The gene for histone RNA hairpin binding protein is located on human chromosome 4 and encodes a novel type of RNA binding protein. EMBO J. 16: 769-778, 1997. 2. Tan, D., Marzluff, W. F., Dominski, Z., Tong, L. Structure of histone mRNA stem-loop, human stem-loop binding protein, and 3-prime-hExo ternary complex. Science 339: 318-321, 2013. 3. Wang, Z.-F., Whitfield, M. L., Ingledue, T. C., III, Dominski, Z., Marzluff, W. F. The protein that binds the 3-prime end of histone mRNA: a novel RNA-binding protein required for histone pre-mRNA processing. Genes Dev. 10: 3028-3040, 1996.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.