Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

Anti-ZAP70 Antibody Picoband® (monoclonal, 6F12) Fluoro488 Conjugated

Selected Variant

M00754-4-Fluoro488

Size: 100 µg/Vial

Product Specifications

Background

ZAP-70 (Zeta-chain-associated protein kinase 70) encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.

Synonyms

Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein; Syk-related tyrosine kinase; ZAP70; SRK

Gene Name

ZAP70

Gene ID

7535

UniProt

P43403

Host

Mouse

Reactivity

Human, Mouse

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human ZAP70, different from the related mouse sequence by two amino acids.

Clonality

Monoclonal

Clone

Clone: 6F12

Tissue Specificity

Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level) . Isoform 3 is expressed in lung, pancreas and spleen (at protein level) . Isoform 4 is expressed in lung and pancreas (at protein level) .

Applications

Flow Cytometry

Field of Research

Diabetes-associated, Heart Disease, Metabolism, Protein Phosphorylation, Ser/Thr Kinases, Serine/Threonine Kinases, Signal Transduction

Purification

Immunogen affinity purified.

Form

Liquid

Function

The major proteolytic product p15 BID allows the release of cytochrome c. Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.

References & Citations

1. Lupher ML, Reedquist KA, Miyake S, Langdon WY, Band H (Sep 1996) . A novel phosphotyrosine-binding domain in the N-terminal transforming region of Cbl interacts ly and selectively with ZAP-70 in T cells.The Journal of Biological Chemistry 271 (39) : 24063–8. 2. Meng W, Sawasdikosol S, Burakoff SJ, Eck MJ (Mar 1999) . Structure of the amino-terminal domain of Cbl complexed to its binding site on ZAP-70 kinase. Nature 398 (6722) : 84–90. 3. Neumeister EN, Zhu Y, Richard S, Terhorst C, Chan AC, Shaw AS (Jun 1995) . Binding of ZAP-70 to phosphorylated T-cell receptor zeta and eta enhances its autophosphorylation and generates specific binding sites for SH2 domain-containing proteins. Molecular and Cellular Biology 15 (6) : 3171–8.

Storage Conditions

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Tyrosine-protein kinase ZAP-70

Subcellular Location

Mitochondrion membrane. Mitochondrion outer membrane. Cytoplasm.

Protein Name

Zeta chain of T cell receptor associated protein kinase 70

Isotype

Mouse IgG2b

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

Price

Sign In for Pricing

Frequently Asked Questions

More Discoveries

Explore Other Products

Browse additional items from our catalog

DOG-1 / TMEM16A (Gastrointestinal Stromal Tumor Marker) (DG1/2564R), CF640R conjugate, 0.1mg/mL
BNC402564-500 1x 500 µL

DOG-1 / TMEM16A (Gastrointestinal Stromal Tumor Marker) (DG1/2564R), CF640R conjugate, 0.1mg/mL

Sign In for Pricing
View Details
CDX2 / Caudal Type Homeobox 2 (GI Epithelial Marker) (rCDX2/1690), CF647 conjugate, 0.1mg/mL
BNC473632-500 1x 500 µL

CDX2 / Caudal Type Homeobox 2 (GI Epithelial Marker) (rCDX2/1690), CF647 conjugate, 0.1mg/mL

Sign In for Pricing
View Details
Factor XIIIa (Coagulation Factor XIIIA Chain) (F13A1/3772R), CF405S conjugate, 0.1mg/mL
BNC043772-500 1x 500 µL

Factor XIIIa (Coagulation Factor XIIIA Chain) (F13A1/3772R), CF405S conjugate, 0.1mg/mL

Sign In for Pricing
View Details
CD20 / MS4A1 (B-Cell Marker) (MS4A1/3410), CF594 conjugate, 0.1mg/mL
BNC943410-500 1x 500 µL

CD20 / MS4A1 (B-Cell Marker) (MS4A1/3410), CF594 conjugate, 0.1mg/mL

Sign In for Pricing
View Details
GFAP (GA-5), CF640R conjugate, 0.1mg/mL
BNC400167-100 1x 100 µL

GFAP (GA-5), CF640R conjugate, 0.1mg/mL

Sign In for Pricing
View Details
Cytokeratin 8 (H1 + TS1), CF594 conjugate, 0.1mg/mL
BNC940687-100 1x 100 µL

Cytokeratin 8 (H1 + TS1), CF594 conjugate, 0.1mg/mL

Sign In for Pricing
View Details