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Anti-ZAP70 Antibody Picoband® (monoclonal, 6F12)

Boster Bio Anti-ZAP70 Antibody Picoband® (monoclonal, 6F12) catalog # M00754-4. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

ZAP-70 (Zeta-chain-associated protein kinase 70) encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.

Synonyms

Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein; Syk-related tyrosine kinase; ZAP70; SRK

Gene Name

ZAP70

Gene ID

7535

UniProt

P43403

Host

Mouse

Reactivity

Human, Mouse

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human ZAP70, different from the related mouse sequence by two amino acids.

Clonality

Monoclonal

Clone

Clone: 6F12

Tissue Specificity

Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level) . Isoform 3 is expressed in lung, pancreas and spleen (at protein level) . Isoform 4 is expressed in lung and pancreas (at protein level) .

Applications

Flow Cytometry, WB

Field of Research

Diabetes-associated, Heart Disease, Metabolism, Protein Phosphorylation, Ser/Thr Kinases, Serine/Threonine Kinases, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Function

The major proteolytic product p15 BID allows the release of cytochrome c. Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.

References & Citations

1. Lupher ML, Reedquist KA, Miyake S, Langdon WY, Band H (Sep 1996) . A novel phosphotyrosine-binding domain in the N-terminal transforming region of Cbl interacts ly and selectively with ZAP-70 in T cells.The Journal of Biological Chemistry 271 (39) : 24063–8. 2. Meng W, Sawasdikosol S, Burakoff SJ, Eck MJ (Mar 1999) . Structure of the amino-terminal domain of Cbl complexed to its binding site on ZAP-70 kinase. Nature 398 (6722) : 84–90. 3. Neumeister EN, Zhu Y, Richard S, Terhorst C, Chan AC, Shaw AS (Jun 1995) . Binding of ZAP-70 to phosphorylated T-cell receptor zeta and eta enhances its autophosphorylation and generates specific binding sites for SH2 domain-containing proteins. Molecular and Cellular Biology 15 (6) : 3171–8.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

70 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Tyrosine-protein kinase ZAP-70

Subcellular Location

Mitochondrion membrane. Mitochondrion outer membrane. Cytoplasm.

Protein Name

Zeta chain of T cell receptor associated protein kinase 70

Isotype

Mouse IgG2b

Contents

243

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