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Anti-Beta Tubulin Antibody Picoband® (monoclonal, 5E4)

Boster Bio Anti-Beta Tubulin Antibody Picoband® (monoclonal, 5E4) catalog # M01857-3. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Tubulin beta chain is a protein that in humans is encoded by the TUBB gene. This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13.

Gene Name

TUBB

Gene ID

203068

UniProt

P07437

Host

Mouse

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Beta Tubulin, identical to the related mouse and rat sequences.

Clonality

Monoclonal

Clone

Clone: 5E4

Tissue Specificity

Widely expressed in fetal and adult tissues.

Applications

Flow Cytometry, IF, ICC, WB

Field of Research

Actin Binding Proteins, Actin, etc., Cytoskeleton, Cytoskeleton/ECM, Microfilaments, Organelle Proteins, Protein Trafficking, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Function

Involved in redox regulation of the cell. Can reduce H (2) O (2) and short chain organic, fatty acid and phospholipid hydroperoxides.May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.

References & Citations

1. Entrez Gene: TUBB tubulin, beta. 2. Volz A, Weiss E, Trowsdale J, Ziegler A (Jan 1994) . Presence of an expressed beta-tubulin gene (TUBB) in the HLA class I region may provide the genetic basis for HLA-linked microtubule dysfunction. Human Genetics. 93 (1) : 42–6.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

25035 MW

Observed Molecular Weight

55 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Subcellular Location

Cytoplasm . Lysosome . Cytoplasmic vesicle . Also found in lung secretory organelles.

Isotype

Mouse IgG1

Contents

243

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