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Anti-DHX15/prp43 Antibody Picoband®

Boster Bio Anti-DHX15/prp43 Antibody Picoband® catalog # A08140-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 is an enzyme that in humans is encoded by the DHX15 gene. It is mapped to 4p15.2. The protein encoded by this gene is a putative ATP-dependent RNA helicase implicated in pre-mRNA splicing.

Synonyms

Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15; ATP-dependent RNA helicase #46; DEAH box protein 15; DHX15; DBP1, DDX15

Gene Name

DHX15

Gene ID

1665

UniProt

O43143

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human DHX15/prp43, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Tissue Specificity

Ubiquitous.

Applications

IF, IHC, ICC, WB, Flow Cytometry

Field of Research

Co-Factors, Epigenetics and Nuclear Signaling, Mediator Complex, Pol II Transcription, Polymerase Associated Factors, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA. In cooperation with TFIP11 seem to be involved in the transition of the U2, U5 and U6 snRNP-containing IL complex to the snRNP-free IS complex leading to efficient debranching and turnover of excised introns.

References & Citations

1. Lüking A, Stahl U, Schmidt U (1998) . The protein family of RNA helicases. Crit. Rev. Biochem. Mol. Biol. 33 (4) : 259–96. 2. Will CL, Schneider C, Hossbach M, et al. (2004) . The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome. RNA. 10 (6) : 929–41. 3. Ota T, Suzuki Y, Nishikawa T, et al. (2004) . Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat. Genet. 36 (1) : 40–5.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

17136 MW

Observed Molecular Weight

91 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

DEAH-box helicase 15

Subcellular Location

Postsynaptic density. Cytoskeleton. Cytoplasm. Axon. Dendrite. Synapse.

Protein Name

Chromaffin granule amine transporter

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

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