Anti-Acetyl Coenzyme A Carboxylase/ACACB Antibody Picoband® Fluoro488 Conjugated

Acetyl-CoA carboxylase 2 also known as ACC-beta or ACC2 is an enzyme that in humans is encoded by the ACACB gene. It is mapped to 12q24.11. Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. ACC-beta is thought to control fatty acid oxidation by means of the ability of malonyl-CoA to inhibit carnitine-palmitoyl-CoA transferase I, the rate-limiting step in fatty acid uptake and oxidation by mitochondria. ACC-beta may be involved in the regulation of fatty acid oxidation, rather than fatty acid biosynthesis. There is evidence for the presence of two ACC-beta isoforms.

A synthetic peptide corresponding to a sequence at the C-terminus of human Acetyl Coenzyme A Carboxylase/ACACB, which shares 64.7% amino acid (aa) sequence identity with mouse Acetyl Coenzyme A Carboxylase/ACACB.

Widely expressed with highest levels in heart, skeletal muscle, liver, adipose tissue, mammary gland, adrenal gland and colon (PubMed:9099716) . Isoform 3 is expressed in skeletal muscle, adipose tissue and liver (at protein level) (PubMed:19190759) . Isoform 3 is detected at high levels in adipose tissue with lower levels in heart, liver, skeletal muscle and testis (PubMed:19190759) .

Catalyzes the ATP-dependent carboxylation of acetyl-CoA to malonyl-CoA (PubMed:20457939, PubMed:19236960, PubMed:20952656) . Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase (PubMed:20457939, PubMed:19236960, PubMed:20952656) . Involved in inhibition of fatty acid and glucose oxidation and enhancement of fat storage (By similarity) . May play a role in regulation of mitochondrial fatty acid oxidation through malonyl-CoA-dependent inhibition of carnitine palmitoyltransferase 1 (By similarity) .

1. Widmer J, Fassihi KS, Schlichter SC, Wheeler KS, Crute BE, King N, Nutile-McMenemy N, Noll WW, Daniel S, Ha J, Kim KH, Witters LA (June 1996) . Identification of a second human acetyl-CoA carboxylase gene. The Biochemical Journal. 316 (Pt 3) (3) : 915–22. 2. Corbett JW, Harwood JH (November 2007) . Inhibitors of mammalian acetyl-CoA carboxylase. Recent Patents on Cardiovascular Drug Discovery. 2 (3) : 162–80. 3. Olson DP, Pulinilkunnil T, Cline GW, Shulman GI, Lowell BB (April 2010) . Gene knockout of Acc2 has little effect on body weight, fat mass, or food intake. Proceedings of the National Academy of Sciences of the United States of America. 107 (16) : 7598–603.