Anti-SKP1 Antibody Picoband®

Skp1 (S-phase kinase-associated protein 1) is a small protein of approximately 160 amino acids. It is mapped to 5q31.1. This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds ly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein (s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7.

S-phase kinase-associated protein 1; Cyclin-A/CDK2-associated protein p19; P19a; Organ of Corti protein 2; OCP-2; Organ of Corti protein II; OCP-II; RNA polymerase II elongation factor-like protein; SIII; Transcription elongation factor B polypeptide 1-like; p19skp1; SKP1; EMC19, OCP2, SKP1A, TCEB1L

Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF (BTRC) and SCF (FBXW11) ubiquitination of CTNNB1 and participate in Wnt signaling. SCF (FBXW11) s ubiquitination of phosphorylated NFKBIA. SCF (BTRC) s ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5, CEP68 and probably NFKB2 (PubMed:25704143) . SCF (SKP2) s ubiquitination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF (SKP2) s ubiquitination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF (FBXW7) s ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF (FBXW2) s ubiquitination of GCM1. SCF (FBXO32) s ubiquitination of MYOD1. SCF (FBXO7) s ubiquitination of BIRC2 and DLGAP5. SCF (FBXO33) s ubiquitination of YBX1. SCF (FBXO11) s ubiquitination of BCL6 and DTL but does not seem to ubiquitination of TP53. SCF (BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF (CCNF) s ubiquitination of CCP110. SCF (FBXL3) and SCF (FBXL21) ubiquitination of CRY1 and CRY2. SCF (FBXO9) s ubiquitination of TTI1 and TELO2. SCF (FBXO10) s ubiquitination of BCL2.

1. Arai, T., Kasper, J. S., Skaar, J. R., Ali, S. H., Takahashi, C., DeCaprio, J. A. Targeted disruption of p185/Cul7 gene results in abnormal vascular morphogenesis. Proc. Nat. Acad. Sci. 100: 9855-9860, 2003. 2. Bai, C., Sen, P., Hofmann, K., Ma, L., Goebl, M., Harper, J. W., Elledge, S. J. SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box. Cell 86: 263-274, 1996. 3. Chen, H., Thalmann, I., Adams, J. C., Avraham, K. B., Copeland, N. G., Jenkins, N. A., Beier, D. R., Corey, D. P., Thalmann, R., Duyk, G. M. cDNA cloning, tissue distribution and chromosomal localization of Ocp2, a gene encoding a putative transcription-associated factor predominantly expressed in the auditory organs. Genomics 27: 389-398, 1995.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.