Anti-Polycystin 2/PKD2 Antibody Picoband® Fluoro647 Conjugated

Polycystin-2?is a?protein?that in humans is encoded by the?PKD2?gene. It is mapped to 4q22.1. This gene encodes a member of the polycystin protein family. The encoded protein is a multi-pass membrane protein that functions as a calcium permeable cation channel, and is involved in calcium transport and calcium signaling in renal epithelial cells. This protein interacts with polycystin 1, and they may be partners in a common signaling cascade involved in tubular morphogenesis. Mutations in this gene are associated with autosomal dominant polycystic kidney disease type 2.

Polycystin-2; PC2; Autosomal dominant polycystic kidney disease type II protein; Polycystic kidney disease 2 protein; Polycystwin; R48321; Transient receptor potential cation channel subfamily P member 2; PKD2; TRPP2

Detected in fetal and adult kidney (PubMed:10770959) . Detected at the thick ascending limb of the loop of Henle, at distal tubules, including the distal convoluted tubule and cortical collecting tubules, with weak staining of the collecting duct (PubMed:10770959) . Detected on placenta syncytiotrophoblasts (at protein level) (PubMed:26269590) . Strongly expressed in ovary, fetal and adult kidney, testis, and small intestine. Not detected in peripheral leukocytes.

Component of a heteromeric calcium-permeable ion channel formed by PKD1 and PKD2 that is activated by interaction between PKD1 and a Wnt family member, such as WNT3A and WNT9B (PubMed:27214281) . Can also form a functional, homotetrameric ion channel (PubMed:29899465) . Functions as a cation channel involved in fluid-flow mechanosensation by the primary cilium in renal epithelium (PubMed:18695040) . Functions as outward-rectifying K+channel, but is also permeable to Ca2+, and to a much lesser degree also to Na+ (PubMed:11854751, PubMed:15692563, PubMed:27071085, PubMed:27991905) . May contribute to the release of Ca2+stores from the endoplasmic reticulum (PubMed:11854751, PubMed:20881056) . Together with TRPV4, forms mechano- and thermosensitive channels in cilium (PubMed:18695040) . PKD1 and PKD2 may function through a common signaling pathway that is necessary to maintain the normal, differentiated state of renal tubule cells. Acts as a regulator of cilium length, together with PKD1. The dynamic control of cilium length is essential in the regulation of mechanotransductive signaling. The cilium length response creates a negative feedback loop whereby fluid shear-mediated deflection of the primary cilium, which decreases intracellular cAMP, leads to cilium shortening and thus decreases flow-induced signaling. Also involved in left-right axis specification via its role in sensing nodal flow; forms a complex with PKD1L1 in cilia to facilitate flow detection in left-right patterning. Detection of asymmetric nodal flow gives rise to a Ca2+signal that is required for normal, asymmetric expression of genes involved in the specification of body left-right laterality (By similarity) .

1. Peters DJ, Spruit L, Saris JJ, Ravine D, Sandkuijl LA, Fossdal R, Boersma J, van Eijk R, Norby S, Constantinou-Deltas CD, et al. (March 1994) . Chromosome 4 localization of a second gene for autosomal dominant polycystic kidney disease. Nat Genet. 5 (4) : 359–62. 2. Li, Qiang; Shen Patrick Y; Wu Guanqing; Chen Xing-Zhen (January 2003) . Polycystin-2 interacts with troponin I, an angiogenesis inhibitor. Biochemistry. United States. 42 (2) : 450–7. 3. Tsiokas, L; Arnould T; Zhu C; Kim E; Walz G; Sukhatme V P (March 1999) . Specific association of the gene product of PKD2 with the TRPC1 channel. Proc. Natl. Acad. Sci. U.S.A. UNITED STATES. 96 (7) : 3934–9. Bibcode:1999PNAS...96.3934T.