Anti-Metabotropic Glutamate Receptor 5/GRM5 Antibody Picoband®

Metabotropic glutamate receptor 5?is a?G protein-coupled receptor?that in humans is encoded by the?GRM5 gene. It is mapped to 11q14.2-q14.3. This gene encodes a member of the G-protein coupled receptor 3 protein family. The encoded protein is a metabatropic glutamate receptor, whose signaling activates a phosphatidylinositol-calcium second messenger system. This protein may be involved in the regulation of neural network activity and synaptic plasticity. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. A pseudogene of this gene has been defined on chromosome 11. Alternative splicing results in multiple transcript variants.

Isoform 1 and isoform 2 are detected in bone marrow cells, spermatogonia and spermatocytes, but not in round spermatids, elongating spermatids and spermatozoa. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa, but not in spermatogonia and spermatocytes (at protein level) . Isoform 1 is widely expressed and detected in fetal liver and bone marrow. Isoform 3 is detected in bone marrow cells enriched in hematopoietic stem cells.

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity.

1. Minakami R, Katsuki F, Yamamoto T, Nakamura K, Sugiyama H (March 1994) . Molecular cloning and the functional expression of two isoforms of human metabotropic glutamate receptor subtype 5. Biochemical and Biophysical Research Communications. 199 (3) : 1136–43. 2. Chen Y, Goudet C, Pin JP, Conn PJ (March 2008) . N-{4-Chloro-2-[ (1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl) methyl]phenyl}-2-hydroxybenzamide (CPPHA) acts through a novel site as a positive allosteric modulator of group 1 metabotropic glutamate receptors. Molecular Pharmacology. 73 (3) : 909–18. 3. Watkins JC, Jane DE (January 2006) . The glutamate story. British Journal of Pharmacology. 147 Suppl 1 (Suppl 1) : S100–8. doi:10.1038/sj.bjp.0706444.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.