Anti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband®

Cytochrome P450 2E1 (abbreviated CYP2E1), a member of the cytochrome P450 mixed-function oxidase system, is involved in the metabolism of xenobiotics in the body. In humans, the CYP2E1 enzyme is encoded by the CYP2E1 gene. It is mapped to 10q26.3. While it is involved in the oxidative metabolism of a small range of substrates (mostly small polar molecules), there are many important drug interactions mediated by CYP2E1. Most drugs undergo deactivation by CYP2E1, either ly or by facilitated excretion from the body. Also, many substances are bioactivated by CYP2E1 to form their active compounds. In addition, CYP2E1 is an important enzyme for the conversion of ethanol to acetaldehyde and to acetate in humans. In the conversion sequence of acetyl-CoA to glucose, CYP2E1 transforms acetone via acetol into propylene glycol and methylglyoxal, the precursors of pyruvate, acetate and lactate.

Expressed in natural killer (NK) cells, CD8 (+) alpha-beta and gamma-delta T-cells. Expressed on essentially all CD56+CD3- NK cells from freshly isolated PBMC. Expressed in interferon-producing killer dendritic cells (IKDCs) .

Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms.

1. Kölble K (December 1993) . Regional mapping of short tandem repeats on human chromosome 10: cytochrome P450 gene CYP2E, D10S196, D10S220, and D10S225. Genomics 18 (3) : 702–4. 2. Hayashi S, Watanabe J, Kawajiri K (October 1991) . Genetic polymorphisms in the 5'-flanking region change transcriptional regulation of the human cytochrome P450IIE1 gene. J. Biochem. 110 (4) : 559–65. 3. Glew, Robert H. You Can Get There From Here: Acetone, Anionic Ketones and Even-Carbon Fatty Acids can Provide Substrates for Gluconeogenesis. Retrieved August 2013.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.