Anti-RNF169 Antibody Picoband® Fluoro550 Conjugated
Product Specifications
Background
RING finger protein 169 (RNF169) is a probable E3 ubiquitin-protein ligase that acts as a negative regulator of the repair of double-strand breaks (DSBs) following DNA damage. RNF169 is recruited to DSB repair sites by recognizing and binding ubiquitin catalyzed by RNF168 and competes with TP53BP1 and BRCA1 for association with RNF168-modified chromatin, thereby acting as a negative regulator of DSB repair. This gene is mapped to 11q13.4.
Synonyms
E3 ubiquitin-protein ligase RNF169; RING finger protein 169; RING-type E3 ubiquitin transferase RNF169; RNF169; KIAA1991
Gene Name
RNF169
Gene ID
254225
UniProt
Q8NCN4
Host
Rabbit
Reactivity
Human, Mouse, Rat
Cross Reactivity
No cross-reactivity with other proteins.
Immunogen
E.coli-derived human RNF169 recombinant protein (Position: N311-K708) .
Clonality
Polyclonal
Tissue Specificity
Expressed in immature but not mature T-cells. Also found in CD34+ cells from peripheral blood, CD34+ precursors from umbilical cord blood and adult bone marrow.
Applications
Flow Cytometry
Field of Research
Hematopoietic Progenitors, Lymphoid, Stem Cells, T Lymphocytic Lineage
Purification
Immunogen affinity purified.
Form
Liquid
Function
Probable E3 ubiquitin-protein ligase that acts as a negative regulator of double-strand breaks (DSBs) repair following DNA damage. Recruited to DSB repair sites by recognizing and binding ubiquitin catalyzed by RNF168 and competes with TP53BP1 and BRCA1 for association with RNF168-modified chromatin, thereby acting as a negative regulator of DSBs repair. E3 ubiquitin-protein ligase activity is not required for regulation of DSBs repair.
References & Citations
1. Chen J, Feng W, Jiang J, Deng Y, Huen MS. Ring finger protein RNF169 antagonizes the ubiquitin-dependent signaling cascade at sites of DNA damage. J Biol Chem. 2012 Aug 10;287 (33) :27715-22. doi: 10.1074/jbc.M112.373530. Epub 2012 Jun 25.
Storage Conditions
At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Calculated Molecular Weight
41409 MW
Specificity
No cross reactivity with other proteins.
Applications Notes
6
Gene Name Synonym
Ring finger protein 169
Subcellular Location
Nucleoplasm.
Protein Name
Protein NDRG3
Isotype
Rabbit IgG
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
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