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Anti-Cytochrome P450 2A6/CYP2A6 Antibody Picoband® Fluoro488 Conjugated

Selected Variant

A00947-2-Fluoro488

Size: 100 µg/Vial

Product Specifications

Background

Cytochrome P450 2A6 (abbreviated CYP2A6) is a member of the cytochrome P450 mixed-function oxidase system, which is involved in the metabolism of xenobiotics in the body. CYP2A6 is the primary enzyme responsible for the oxidation of nicotine and cotinine. It is also involved in the metabolism of several pharmaceuticals, carcinogens, and a number of coumarin-type alkaloids. CYP2A6 is the only enzyme in the human body that appreciably catalyzes the 7-hydroxylation of coumarin, such that the formation of the product of this reaction, 7-hydroxycoumarin, is used as a probe for CYP2A6 activity. The CYP2A6 gene is part of a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. The gene was formerly referred to as CYP2A3; however, it has been renamed CYP2A6.

Synonyms

Cytochrome P450 2A6; 1,4-cineole 2-exo-monooxygenase; CYPIIA6; Coumarin 7-hydroxylase; Cytochrome P450 IIA3; Cytochrome P450 (I) ; CYP2A6; CYP2A3

Gene Name

CYP2A6

Gene ID

1548

UniProt

P11509

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Cytochrome P450 2A6/CYP2A6.

Clonality

Polyclonal

Tissue Specificity

Liver.

Applications

Flow Cytometry

Field of Research

Cell Differentiation, Developmental Biology, Epigenetics and Nuclear Signaling, Hematopoietic Progenitors, Organogenesis, Stem Cells

Purification

Immunogen affinity purified.

Form

Liquid

Function

Exhibits a high coumarin 7-hydroxylase activity. Can act in the hydroxylation of the anti-cancer drugs cyclophosphamide and ifosphamide. Competent in the metabolic activation of aflatoxin B1. Constitutes the major nicotine C-oxidase. Acts as a 1,4-cineole 2-exo-monooxygenase. Possesses low phenacetin O-deethylation activity.

References & Citations

1. Entrez Gene: CYP2A6 cytochrome P450, family 2, subfamily A, polypeptide 6. 2. Wen X, Wang JS, Neuvonen PJ, Backman JT (2002) . Isoniazid is a mechanism-based inhibitor of cytochrome P450 1A2, 2A6, 2C19 and 3A4 isoforms in human liver microsomes. Eur. J. Clin. Pharmacol. 57 (11) : 799–804.

Storage Conditions

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Calculated Molecular Weight

50408 MW

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Cytochrome P450 family 2 subfamily A member 6

Subcellular Location

Endoplasmic reticulum membrane. Peripheral membrane protein. Microsome membrane. Peripheral membrane protein

Protein Name

Protein C-ets-1

Isotype

Rabbit IgG

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

Price

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