Anti-CD40L/CD40LG Antibody Picoband® Fluoro594 Conjugated

CD40 ligand (CD40L) is a type II membrane protein of 261 amino acids on activated T cells that induces B cell proliferation and immunoglobulin secretion. It has homology with tumour necrosis factor-alpha and -beta, and has important functions in B-cell activation and differentiation. Human CD40L with 5 exons, is mapped to the proximal region of the mouse X chromosome on Xq26.3-27.1, and can be detected on T cells but is absent from B cells and monocytes. Since CD40L is expressed on platelets and released from them on activation, its predictive value as a marker for clinical outcome and the therapeutic effect of inhibition of glycoprotein IIb /IIIa receptor in patients with acute coronary syndromes was investigated. The soluble CD40L may be involved in the process of restenosis and that it exerts its effect by triggering a complex group of inflammatory reactions on endothelial and mononuclear cells.CD40L plays a central role in the pathophysiology of acute coronary syndromes, and has a role in the pathogenesis of coronary artery lesions.

CD40 ligand; CD40-L; T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5; CD154; CD40LG; CD40L; TNFSF5; TRAP

A synthetic peptide corresponding to a sequence in the middle region of human CD40L/CD40LG, which shares 82.8% and 79.3% amino acid (aa) sequence identity with mouse and rat CD40L/CD40LG, respectively.

Adaptive Immunity, Atherosclerosis, Cardiovascular, Cytokines, Immunoglobulins, Immunology, Innate Immunity, Platelets, T Cells, TNF Superfamily, Vascular Inflammation

Cytokine that binds to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells. Induces tyrosine phosphorylation of isoform 3 of CD28. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching (By similarity) . Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and a matrix metalloproteinases (MMP) inhibitor-sensitive pathway.

1. Allen, R.C.; Armitage, R.J.; Conley, M.E.; Rosenblatt, H.; Jenkins, N.A.; Copeland, N.G.; Bedell, M.A.; Edelhoff, S.; Disteche, C.M.; Simoneaux, D.K.; Fanslow, W.C.; Belmont, J.; Spriggs, M.K.:CD40 ligand gene defects responsible for X-linked hyper-IgM syndrome. Science 259: 990-993, 1993. 2. Cipollone, F.; Ferri, C.; Desideri, G.; Paloscia, L.; Materazzo, G.; Mascellanti, M.; Fazia, M.; Iezzi, A.; Cuccurullo, C.; Pini, B.; Bucci, M.; Santucci, A.; Cuccurullo, F.; Mezzetti, A.:Preprocedural level of soluble CD40L is predictive of enhanced inflammatory response and restenosis after coronary angioplasty. Circulation 108: 2776-2782, 2003.