Anti-ZP3 Antibody Picoband® Fluoro488 Conjugated

Zona pellucida sperm-binding protein 3, also known as zona pellucida glycoprotein 3 (Zp-3) or the sperm receptor, is a ZP module-containing protein that in humans is encoded by the ZP3 gene. It is mapped to 7q11.23. he zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in primary binding and induction of the sperm acrosome reaction. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a C-terminal consensus furin cleavage site, and a transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus; however, sequence and literature review reveals that there is only one full-length ZP3 locus in the human genome. Another locus encoding a bipartite transcript designated POMZP3 contains a duplication of the last four exons of ZP3, including the above described variation, and maps closely to this gene.

A synthetic peptide corresponding to a sequence in the middle region of human ZP3, which shares 77.4% amino acid (aa) sequence identity with both mouse and rat ZP3.

The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.

1. Chen, T., Bian, Y., Liu, X., Zhao, S., Wu, K., Yan, L., Li, M., Yang, Z., Liu, H., Zhao, H., Chen, Z.-J. A recurrent missense mutation in ZP3 causes empty follicle syndrome and female infertility. Am. J. Hum. Genet. 101: 459-465, 2017. 2. Dean, J. Biology of mammalian fertilization: role of the zona pellucida. J. Clin. Invest. 89: 1055-1059, 1992. 3. Kipersztok, S., Osawa, G. A., Liang, L. F., Modi, W. S., Dean, J. POM-ZP3, a bipartite transcript derived from human ZP3 and POM121 homologue. Genomics 25: 354-359, 1995.