Anti-WEE1 Antibody Picoband® Fluoro488 Conjugated
Product Specifications
Background
Wee1 is a nuclear kinase belonging to the Ser/Thr family of protein kinases in the fission yeast Schizosaccharomyces pombe (S. pombe) . I t is mapped to 11p15.4. This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.
Synonyms
Wee1-like protein kinase; WEE1hu; Wee1A kinase; WEE1
Gene Name
WEE1
Gene ID
7465
UniProt
P30291
Host
Rabbit
Reactivity
Human, Mouse, Rat
Cross Reactivity
No cross-reactivity with other proteins.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human WEE1, which shares 94.4% and 91.7% amino acid (aa) sequence identity with mouse and rat WEE1, respectively.
Clonality
Polyclonal
Tissue Specificity
Predominantly expressed in T-cells. Also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium.
Applications
Flow Cytometry
Field of Research
Cell Biology, Cell Cycle, Epigenetics and Nuclear Signaling, Kinases/Phosphatases
Purification
Immunogen affinity purified.
Form
Liquid
Function
Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
References & Citations
1. Heald, R., McLoughlin, M., McKeon, F. Human wee1 maintains mitotic timing by protecting the nucleus from cytoplasmically activated cdc2 kinase. Cell 74: 463-474, 1993. 2. Igarashi, M., Nagata, A., Jinno, S., Suto, K., Okayama, H. Wee1 (+) -like gene in human cells. Nature 353: 80-83, 1991. 3. Matsuo, T., Yamaguchi, S., Mitsui, S., Emi, A., Shimoda, F., Okamura, H. Control mechanism of the circadian clock for timing of cell division in vivo. Science 302: 255-259, 2003.
Storage Conditions
At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Calculated Molecular Weight
60066 MW
Specificity
No cross reactivity with other proteins.
Applications Notes
6
Gene Name Synonym
WEE1 G2 checkpoint kinase
Subcellular Location
Nucleus.
Protein Name
Heparanase
Isotype
Rabbit IgG
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
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