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Anti-TMEM166/EVA1A Antibody Picoband®

Boster Bio Anti-TMEM166/EVA1A Antibody Picoband® catalog # A11580-1. Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Eva-1 homolog A (C. elegans) is a protein that in humans is encoded by the EVA1A gene. It belongs to the FAM176 family. This gene is mapped to 2p12. EVA1A, also called TMEM166, acts as a regulator of programmed cell death, mediating both autophagy and apoptosis.

Synonyms

Protein eva-1 homolog A; Protein FAM176A; Transmembrane protein 166; EVA1A; FAM176A; TMEM166; SP24

Gene Name

EVA1A

Gene ID

84141

UniProt

Q9H8M9

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human TMEM166/EVA1A, which shares 66.7% and 50% amino acid (aa) sequence identity with mouse and rat TMEM166/EVA1A, respectively.

Clonality

Polyclonal

Tissue Specificity

Expressed in lung, kidney, liver, pancreas, placenta, but not in heart and skeletal muscle.

Applications

Flow Cytometry, IHC, IHC-F, ICC, WB

Field of Research

Cell Biology, Proteasome / Ubiquitin, Proteolysis/Ubiquitin, Ubiquitin E3 Enzymes

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Acts as a regulator of programmed cell death, mediating both autophagy and apoptosis.

References & Citations

1. Sun W, Ma XM, Bai JP, Zhang GQ, Zhu YJ, Ma HM, Guo H, Chen YY, Ding JB (2012) . Transmembrane protein 166 expression in esophageal squamous cell carcinoma in Xinjiang, China. Asian Pacific Journal of Cancer Prevention. 13 (8) : 3713–6. 2. Wang L, Yu C, Lu Y, He P, Guo J, Zhang C, Song Q, Ma D, Shi T, Chen Y (August 2007) . TMEM166, a novel transmembrane protein, regulates cell autophagy and apoptosis. Apoptosis. 12 (8) : 1489–502.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

21 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Eva-1 homolog A, regulator of programmed cell death

Subcellular Location

Endoplasmic reticulum membrane, Single-pass membrane protein, Lysosome membrane.

Isotype

Rabbit IgG

Contents

243

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