Anti-Aprt Antibody Picoband® Fluoro647 Conjugated

Adenine phosphoribosyltransferase (APRTase) is anenzymeencoded by theAPRTgene, found inhumanson chromosome 16. It belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP) . It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene.

A synthetic peptide corresponding to a sequence at the C-terminus of mouse APRT, which shares 86.4% and 95.5% amino acid (aa) sequence identity with human and rat APRT, respectively.

1. Silva CH, Silva M, Iulek J, Thiemann OH (Jun 2008) . Structural complexes of human adenine phosphoribosyltransferase reveal novel features of the APRT catalytic mechanism. Journal of Biomolecular Structure & Dynamics. 25 (6) : 589–97. 2. Sarver AE, Wang CC (Oct 2002) . The adenine phosphoribosyltransferase from Giardia lamblia has a unique reaction mechanism and unusual substrate binding properties. The Journal of Biological Chemistry. 277 (42) : 39973–80. 3. Valaperta R, Rizzo V, Lombardi F, Verdelli C, Piccoli M, Ghiroldi A, Creo P, Colombo A, Valisi M, Margiotta E, Panella R, Costa E (1 July 2014) . Adenine phosphoribosyltransferase (APRT) deficiency: identification of a novel nonsense mutation. BMC Nephrology. 15: 102.