Anti-TCF7 Antibody Picoband® Fluoro647 Conjugated

Transcription factor 7 is a protein that in humans is encoded by the TCF7 gene. It is mapped to 5q31.1. This gene encodes a member of the T-cell factor/lymphoid enhancer-binding factor family of high mobility group (HMG) box transcriptional activators. This gene is expressed predominantly in T-cells and plays a critical role in natural killer cell and innate lymphoid cell development. The encoded protein forms a complex with beta-catenin and activates transcription through a Wnt/beta-catenin signaling pathway. Mice with a knockout of this gene are viable and fertile, but display a block in T-lymphocyte differentiation. Alternative splicing results in multiple transcript variants. Naturally-occurring isoforms lacking the N-terminal beta-catenin interaction domain may act as dominant negative regulators of Wnt signaling.

A synthetic peptide corresponding to a sequence in the middle region of human TCF7, which shares 90.6% amino acid (aa) sequence identity with mouse TCF7.

Predominantly expressed in T-cells. Also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium.

Autoimmune, Domain Families, Epigenetics and Nuclear Signaling, Immune System Diseases, Immunology, Nuclear, Signaling Pathways, Stem Cells, Transcription

Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4+ CD8+ immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1.

1. Driessens, G., Zheng, Y., Gajewski, T. F. Beta-catenin does not regulate memory T cell phenotype. (Letter) Nature Med. 16: 513-514, 2010. 2. Gattinoni, L., Ji, Y., Restifo, N. P. Reply to Driessens et al. (Letter) Nature Med. 16: 514-515, 2010. 3. Gattinoni, L., Zhong, X.-S., Palmer, D. C., Ji, Y., Hinrichs, C. S., Yu, Z., Wrzesinski, C., Boni, A., Cassard, L., Garvin, L. M., Paulos, C. M., Muranski, P., Restifo, N. P. Wnt signaling arrests effector T cell differentiation and generates CD8+ memory stem cells. Nature Med. 15: 808-813, 2009.