Anti-Collagen III/COL3A1 Antibody Picoband® Fluoro647 Conjugated

COL3A1, also called EDS4A or Collagen alpha-1 (III), is a protein that in humans is encoded by the COL3A1 gene. It is mapped to 2q32.2. COL3A1 chain is a fibrillar-forming collagen comprising 3 alpha-1 (III) chains and is expressed in early embryos and throughout embryogenesis. In adult, COL3A1 is a major component of the extracellular matrix in a variety of internal organs and skin. COL3A1 is also a fibrous scleroprotein in bone, cartilage, dentin, tendon, bone marrow stroma and other connective tissue. It is involved in regulation of cortical development, and it is the major ligand of GPR56 in the developing brain. COL3A1 binding to GPR56 can inhibit neuronal migration and activate the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12.

Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.

Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.

1. Janeczko RA, Ramirez F (Oct 1989) . Nucleotide and amino acid sequences of the entire human alpha 1 (III) collagen. Nucleic Acids Res 17 (16) : 6742. 2. Superti-Furga A, Gugler E, Gitzelmann R, Steinmann B (Jun 1988) . Ehlers-Danlos syndrome type IV: a multi-exon deletion in one of the two COL3A1 alleles affecting structure, stability, and processing of type III procollagen. J Biol Chem 263 (13) : 6226–32.