Anti-Anillin/ANLN Antibody Picoband®

Anillin is a conserved protein implicated in cytoskeletal dynamics during cellularization and cytokinesis. This gene is mapped to 7p14.2. The ANLN gene in humans and the scraps gene in Drosophila encode Anillin. The human anillin cDNA, located on Chr7, encodes a 1,125–amino acid protein with a predicted molecular mass of 124 kD and a pI of 8.1. The mouse anillin gene is located on Chr9. This gene encodes an actin-binding protein that plays a role in cell growth and migration, and in cytokinesis. The encoded protein is thought to regulate actin cytoskeletal dynamics in podocytes, components of the glomerulus. Mutations in this gene are associated with focal segmental glomerulosclerosis 8. Alternative splicing results in multiple transcript variants encoding different isoforms.

A synthetic peptide corresponding to a sequence at the C-terminus of human Anillin/ANLN, which shares 96.4% amino acid (aa) sequence identity with both mouse and rat Anillin/ANLN.

Ubiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. In the kidney, it is widely expressed in tubules, but sparsely expressed in the glomerulus (PubMed:24676636) . Expression is significantly increased in renal biopsy specimens from idiopathic FSGS (PubMed:24676636) . Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors.

Actin Binding Proteins, Actin, etc., Cell Biology, Cell Cycle, Cell Division, Cytokinesis, Cytoskeleton, Cytoskeleton/ECM, Microfilaments, Signal Transduction

Required for cytokinesis (PubMed:16040610) . Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression. Plays a role in bleb assembly during metaphase and anaphase of mitosis (PubMed:23870127) . May play a significant role in podocyte cell migration (PubMed:24676636) .

1. Piekny, A. J., & Maddox, A. S. (2010) . The myriad roles of Anillin during cytokinesis. Semin Cell Dev Biol, 21 (9), 881-891. 2. Oegema, K., Savovian, M. S., Mitchison, T. J., & Field, C. M. (2000) . Functional Analysis of a Human Homologue of the Drosophila Actin Binding Protein Anillin Suggest a Role in Cytokinesis. The Journal of Cell Biology, 150 (3), 539-551. 3. D'Avino, P. P., Takeda, T., Capalbo, L., Zhang, W., Lilley, K. S., Laue, E. D., & Glover, D. M. (2008) . Interaction between Anillin and RacGAP50C connects the actomyosin contractile ring with spindle microtubules at the cell division site. J Cell Sci, 121 (Pt 8), 1151-1158.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.