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Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 Antibody Picoband®

Boster Bio Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 Antibody Picoband® catalog # A01981-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Synonyms

Neuronal acetylcholine receptor subunit alpha-3; CHRNA3; NACHRA3

Gene Name

CHRNA3

Gene ID

1136

UniProt

P32297

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human CHRNA3, which shares 97.4% and 94.7% amino acid (aa) sequence identity with mouse and rat CHRNA3, respectively.

Clonality

Polyclonal

Applications

Flow Cytometry, WB

Field of Research

Adaptive Immunity, Immunology, T Cells

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

References & Citations

1. Entrez Gene: CHRNA3 cholinergic receptor, nicotinic, alpha 3. 2. Eng CM, Kozak CA, Beaudet AL, Zoghbi HY (Apr 1991) . Mapping of multiple subunits of the neuronal nicotinic acetylcholine receptor to chromosome 15 in man and chromosome 9 in mouse. Genomics. 9 (2) : 278–82. 3. Mihovilovic M, Roses AD (1991) . Expression of mRNAs in human thymus coding for the alpha 3 subunit of a neuronal acetylcholine receptor.. Exp. Neurol. 111 (2) : 175–80.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

58 kDa

Observed Molecular Weight

50-55 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Cholinergic receptor nicotinic alpha 3 subunit

Subcellular Location

Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

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