Anti-epithelial Sodium Channel alpha/SCNN1A Antibody Picoband® Fluoro488 Conjugated

The SCNN1A gene encodes the alpha subunit of the epithelial sodium channel (ENaC), a constitutively active channel that allows the flow of sodium ions from the lumen into epithelial cells across the apical cell membrane. The ENaC channel, which is regulated by the renin-angiotensin-aldosterone system, has a central role in the regulation of extracellular fluid volume and blood pressure. The other subunits are encoded by the beta (SCNN1B), gamma (SCNN1G), and delta (SCNN1D) genes. This SCNN1A gene is mapped to 12p13.31. Mutations in this gene have been associated with pseudohypoaldosteronism type 1 (PHA1), a rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids.

Amiloride-sensitive sodium channel subunit alpha; Alpha-NaCH; Epithelial Na (+) channel subunit alpha; Alpha-ENaC; ENaCA; Nonvoltage-gated sodium channel 1 subunit alpha; SCNEA; SCNN1A; SCNN1

A synthetic peptide corresponding to a sequence at the C-terminus of human SCNN1A, which shares 83.3% amino acid (aa) sequence identity with both mouse and rat SCNN1A.

Expressed in the female reproductive tract, from the fimbrial end of the fallopian tube to the endometrium (at protein level) (PubMed:22207244) . Expressed in kidney (at protein level) . In the respiratory tract, expressed in the bronchial epithelium (at protein level) . Highly expressed in lung. Detected at intermediate levels in pancreas and liver, and at low levels in heart and placenta (PubMed:22207244) . in skin, expressed in keratinocytes, melanocytes and Merkel cells of the epidermal sub- layers, stratum basale, stratum spinosum and stratum granulosum (at protein level) (PubMed:28130590) . Expressed in the outer root sheath of the hair follicles (at protein level) (PubMed:28130590) . Detected in both peripheral and central cells of the sebaceous gland (at protein level) (PubMed:28130590) . Expressed by eccrine sweat glands (at protein level) (PubMed:28130590) . In skin, also expressed by arrector pili muscle cells and intradermal adipocytes (PubMed:28130590) . Isoform 1 and isoform 2 predominate in all tissues. Expression of isoform 3, isoform 4 and isoform 5 is very low or not detectable, except in lung and heart (PubMed:9575806) .

Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and eccrine sweat glands. Also plays a role in taste perception.

1. Hanukoglu, I., Hanukoglu, A. Epithelial sodium channel (ENaC) family: phylogeny, structure-function, tissue distribution, and associated inherited diseases. Gene 579: 95-132, 2016. 2. Iwai, N., Baba, S., Mannami, T., Ogihara, T., Ogata, J. Association of a sodium channel alpha subunit promoter variant with blood pressure. J. Am. Soc. Nephrol. 13: 80-85, 2002.