Anti-DNA Polymerase iota/POLI Antibody Picoband® Fluoro647 Conjugated

DNA polymerase iota is an enzyme that in humans is encoded by the POLI gene. The protein encoded by this gene is an error-prone DNA polymerase involved in DNA repair. The encoded protein promotes DNA synthesis across lesions in the template DNA, which other polymerases cannot do. The encoded polymerase inserts deoxynucleotides across lesions and then relies on DNA polymerase zeta to extend the nascent DNA strand to bypass the lesion.

A synthetic peptide corresponding to a sequence at the C-terminus of human DNA Polymerase iota, which shares 70.6% and 76.5% amino acid (aa) sequence identity with mouse and rat DNA Polymerase iota, respectively.

Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.

1. Frank EG, Woodgate R (Aug 2007) . Increased catalytic activity and altered fidelity of human DNA polymerase iota in the presence of manganese. J Biol Chem. 282 (34) : 24689–96. 2. McDonald JP, Tissier A, Frank EG, et al. (2001) . DNA polymerase iota and related rad30-like enzymes.. Philos. Trans. R. Soc. Lond., B, Biol. Sci. 356 (1405) : 53–60.