Anti-GPCR GPR40/FFAR1 Antibody Picoband®

Free fatty acid receptor 1 (FFA1), also known as GPR40, is a class A G-protein coupled receptor that in humans is encoded by the FFAR1 gene. This gene encodes a member of the GP40 family of G protein-coupled receptors that are clustered together on chromosome 19. The encoded protein is a receptor for medium and long chain free fatty acids and may be involved in the metabolic regulation of insulin secretion. Polymorphisms in this gene may be associated with type 2 diabetes.

A synthetic peptide corresponding to a sequence at the C-terminus of human GPCR GPR40, which shares 88% and 84% amino acid (aa) sequence identity with mouse and rat GPCR GPR40, respectively.

G-protein coupled receptor for medium and long chain saturated and unsaturated fatty acids that plays an important role in glucose homeostasis. Fatty acid binding increases glucose- stimulated insulin secretion, and may also enhance the secretion of glucagon-like peptide 1 (GLP-1) . May also play a role in bone homeostasis; receptor signaling activates pathways that inhibit osteoclast differentiation (By similarity) . Ligand binding leads to a conformation change that triggers signaling via G-proteins that activate phospholipase C, leading to an increase of the intracellular calcium concentration. Seems to act through a G (q) and G (i) -mediated pathway.

1. Ichimura A, Hirasawa A, Hara T, Tsujimoto G (September 2009) . Free fatty acid receptors act as nutrient sensors to regulate energy homeostasis. Prostaglandins Other Lipid Mediat. 89 (3-4) : 82–8. 2. Sum CS, Tikhonova IG, Neumann S, Engel S, Raaka BM, Costanzi S, Gershengorn MC (October 2007) . Identification of residues important for agonist recognition and activation in GPR40. J. Biol. Chem. 282 (40) : 29248–55.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.