Anti-Cullin 4a/CUL4A Antibody Picoband® Fluoro647 Conjugated

Cullin-4A is a protein that in humans is encoded by the CUL4A gene. It belongs to the cullin family of ubiquitin ligase proteins and is highly homologous to the CUL4B protein. CUL4A regulates numerous key processes such as DNA repair, chromatin remodeling, spermatogenesis, haematopoiesis and the mitotic cell cycle. As a result, CUL4A has been implicated in several cancers and the pathogenesis of certain viruses including HIV. Interestingly, a component of a CUL4A complex, Cereblon, was discovered to be a major target of the teratogenic agent thalidomide.

Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX (DET1-COP1) s ubiquitination of JUN. DCX (DDB2) s ubiquitination of XPC. DCX (DDB2) ubiquitinates histones H3-H4 and is required for efficient histone deposition during replication-coupled (H3.1) and replication-independent (H3.3) nucleosome assembly, probably by facilitating the transfer of H3 from ASF1A/ASF1B to other chaperones involved in histone deposition. DCX (DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9. DCX (DTL) s autoubiquitination of DTL.

1. Entrez Gene: CUL4A Cullin 4A. 2. Liu L, Lee S, Zhang J, Peters SB, Hannah J, Zhang Y, Yin Y, Koff A, Ma L, Zhou P (May 2009) . CUL4A abrogation augments DNA damage response and protection against skin carcinogenesis. Molecular Cell. 34 (4) : 451–60. 3. Shiyanov P, Nag A, Raychaudhuri P (Dec 1999) . Cullin 4A associates with the UV-damaged DNA-binding protein DDB. The Journal of Biological Chemistry. 274 (50) : 35309–12.