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Anti-CEP68 Antibody Picoband®

Boster Bio Anti-CEP68 Antibody Picoband® catalog # A01704-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Centrosomal protein of 68 kDa is a protein that in humans is encoded by the CEP68 gene. It is mapped to chromosome 2. CEP68 is required for centrosome cohesion. It decorates fibres emanating from the proximal ends of centrioles. CEP68 and rootletin depend both on each other for centriole association, and both also require CEP250 for their function.

Synonyms

Centrosomal protein of 68 kDa; Cep68; CEP68; KIAA0582

Gene Name

CEP68

Gene ID

23177

UniProt

Q76N32

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human CEP68, which shares 73.2% and 75.6% amino acid (aa) sequence identity with mouse and rat EP68, respectively.

Clonality

Polyclonal

Tissue Specificity

Present in many fetal and adult tissues. Mainly expressed in adult skeletal muscle, thymus, testis, ovary, and pancreas, low or absent in brain and peripheral blood leukocytes.

Applications

WB

Field of Research

Organelles, Subcellular Markers, Tags & Cell Markers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Involved in maintenance of centrosome cohesion, probably as part of a linker structure which prevents centrosome splitting (PubMed:18042621) . Required for localization of CDK5RAP2 to the centrosome during interphase (PubMed:24554434, PubMed:25503564) .

References & Citations

1. Fang, G., Zhang, D., Yin, H., Zheng, L., Bi, X., Yuan, L. Centlein mediates an interaction between C-Nap1 and Cep68 to maintain centrosome cohesion. J. Cell Sci. 127: 1631-1639, 2014. 2. Graser, S., Stierhof, Y.-D., Nigg, E. A. Cep68 and Cep215 (Cdk5rap2) are required for centrosome cohesion. J. Cell. Sci. 120: 4321-4331, 2007. 3. Man, X., Megraw, T. L., Lim, Y. P. Cep68 can be regulated by Nek2 and SCF complex. Europ. J. Cell Biol. 94: 162-172, 2015.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

81 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Centrosomal protein 68

Subcellular Location

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.

Isotype

Rabbit IgG

Contents

243

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