Anti-RNF186 Antibody Picoband® Fluoro647 Conjugated
Product Specifications
Background
RNF186 is an E3 ubiquitin ligase that regulates endoplasmic reticulum (ER) stress and apoptosis through its interaction with BNIP1. This gene is mapped to chromosome 1p36.13 based on an alignment of theRNF186 sequence with the genomic sequence (GRCh38) . Cotransfection and immunoprecipitation analyses showed that RNF186, through it transmembrane domains, interacted with BNIP1. Overexpression of RNF186 promoted BNIP1 ubiquitination and transfer of BNIP1 to mitochondria.
Synonyms
RING finger protein 186; RNF186
Gene Name
RNF186
Gene ID
54546
UniProt
Q9NXI6
Host
Rabbit
Reactivity
Human
Cross Reactivity
No cross-reactivity with other proteins.
Immunogen
E. coli-derived human RNF186 recombinant protein (Position: R86-L159) .
Clonality
Polyclonal
Tissue Specificity
Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.
Applications
Flow Cytometry
Field of Research
Cell Biology
Purification
Immunogen affinity purified.
Form
Liquid
Function
Required for DNA double-strand breaks (DSBs) formation in unsynapsed regions during meiotic recombination. Probably acts by forming a complex with MEI4 and REC114, which activates DSBs formation in unsynapsed regions, an essential step to ensure completion of synapsis. Not required for HORMAD1 functions in pairing-independent synaptonemal complex formation, ATR recruitment to unsynapsed axes, meiotic silencing of unsynapsed chromatin (MSUC) or meiotic surveillance.
References & Citations
1. Gross, M. B. Personal Communication. Baltimore, Md. 10/18/2016. 2. Wang, P., Wu, Y., Li, Y., Zheng, J., Tang, J. A novel RING finger E3 ligase RNF186 regulates ER stress-mediated apoptosis through interaction with BNip1. Cell. Signal. 25: 2320-2333, 2013.
Storage Conditions
At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Calculated Molecular Weight
24145 MW
Specificity
No cross reactivity with other proteins.
Applications Notes
6
Gene Name Synonym
RING finger protein 186
Subcellular Location
Membrane ; Multi-pass membrane protein .
Protein Name
Transmembrane protein 240
Isotype
Rabbit IgG
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
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