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Anti-Glycerol kinase/GK Antibody Picoband®

Selected Variant

A02884-1

Size: 100 µg/Vial

Boster Bio Anti-Glycerol kinase/GK Antibody Picoband® catalog # A02884-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Glycerol kinase is a phosphotransferase enzyme involved in triglycerides and glycerophospholipids synthesis. The protein encoded by this gene belongs to the FGGY kinase family. This protein is a key enzyme in the regulation of glycerol uptake and metabolism. It catalyzes the phosphorylation of glycerol by ATP, yielding ADP and glycerol-3-phosphate. Mutations in this gene are associated with glycerol kinase deficiency (GKD) . Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Synonyms

Glycerol kinase; GK; Glycerokinase; ATP:glycerol 3-phosphotransferase; GK

Gene Name

GK

Gene ID

2710

UniProt

P32189

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human Glycerol kinase recombinant protein (Position: M1-S180) .

Clonality

Polyclonal

Tissue Specificity

Highly expressed in the liver, kidney and testis. Isoform 2 and isoform 3 are expressed specifically in testis and fetal liver, but not in the adult liver.

Applications

ELISA, IHC, WB

Field of Research

Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Key enzyme in the regulation of glycerol uptake and metabolism.

References & Citations

1. Dipple, K. M., Zhang, Y.-H., Huang, B.-L., McCabe, L. L., Dallongeville, J., Inokuchi, T., Kimura, M., Marx, H. J., Roederer, G. O., Shih, V., Yamaguchi, S., Yoshida, I., McCabe, E. R. B.Glycerol kinase deficiency: evidence for complexity in a single gene disorder. Hum. Genet. 109: 55-62, 2001. 2. Gaudet, D., Arsenault, S., Perusse, L., Vohl, M.-C., St.-Pierre, J., Bergeron, J., Despres, J.-P., Dewar, K., Daly, M. J., Hudson, T., Rioux, J. D. Glycerol as a correlate of impaired glucose tolerance: dissection of a complex system by use of a simple genetic trait. Am. J. Hum. Genet. 66: 1558-1568, 2000.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

61 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Glycerol kinase

Subcellular Location

Mitochondrion outer membrane; In sperm and fetal tissues, the majority of the enzyme is bound to mitochondria, but in adult tissues, such as liver found in the cytoplasm.

Isotype

Rabbit IgG

Contents

243

Price

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