Anti-MED18 Antibody Picoband® Fluoro647 Conjugated
Product Specifications
Background
MED18 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II. Using in vitro-translated epitope-tagged proteins for protein-binding assays, it is found that MED18 ly interacted with the Mediator subunit TRFP (MED20) . The MED18-TRFP heterodimer could also be coimmunoprecipitated from cotransfected insect cells. The MED18 gene was mapped to chromosome 1p35.3 based on an alignment of the MED18 sequence with the genomic sequence.
Synonyms
Mediator of RNA polymerase II transcription subunit 18; Mediator complex subunit 18; p28b; MED18
Gene Name
MED18
Gene ID
54797
UniProt
Q9BUE0
Host
Rabbit
Reactivity
Human, Mouse, Rat
Cross Reactivity
No cross-reactivity with other proteins.
Immunogen
E. coli-derived human MED18 recombinant protein (Position: M1-M208) .
Clonality
Polyclonal
Tissue Specificity
Expressed in immature but not mature T-cells. Also found in CD34+ cells from peripheral blood, CD34+ precursors from umbilical cord blood and adult bone marrow.
Applications
Flow Cytometry
Field of Research
Epigenetics and Nuclear Signaling, Mediator Complex, Transcription
Purification
Immunogen affinity purified.
Form
Liquid
Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
References & Citations
1. Hartz, P. A. Personal Communication. Baltimore, Md. 10/30/2008. 2. Sato, S., Tomomori-Sato, C., Banks, C. A. S., Sorokina, I., Parmely, T. J., Kong, S. E., Jin, J., Cai, Y., Lane, W. S., Brower, C. S., Conaway, R. C., Conaway, J. W. Identification of mammalian mediator subunits with similarities to yeast mediator subunits Srb5, Srb6, Med11, and Rox3. J. Biol. Chem. 278: 15123-15127, 2003.
Storage Conditions
At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Calculated Molecular Weight
10201 MW
Specificity
No cross reactivity with other proteins.
Applications Notes
6
Gene Name Synonym
Mediator complex subunit 18
Subcellular Location
Nucleus .
Protein Name
Neutrophil defensin 1
Isotype
Rabbit IgG
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
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