Anti-RanBP2 Antibody Picoband®

RAN binding protein 2 (RANBP2) is protein which in humans is encoded by the RANBP2 gene. This gene encodes a very large RAN-binding protein that immunolocalizes to the nuclear pore complex. The protein is a giant scaffold and mosaic cyclophilin-related nucleoporin implicated in the Ran-GTPase cycle. And the encoded protein ly interacts with the E2 enzyme UBC9 and strongly enhances SUMO1 transfer from UBC9 to the SUMO1 target SP100. These findings place sumoylation at the cytoplasmic filaments of the nuclear pore complex and suggest that, for some substrates, modification and nuclear import are linked events. This gene is partially duplicated in a gene cluster that lies in a hot spot for recombination on chromosome 2q.

E3 SUMO-protein ligase RanBP2;6.3.2.-;358 kDa nucleoporin; Nuclear pore complex protein Nup358; Nucleoporin Nup358; Ran-binding protein 2; RanBP2; p270; RANBP2; NUP358

Cell Biology, Cell Cycle, Cell Division, DNA/RNA, Epigenetics and Nuclear Signaling, Nuclear Import/Export, Proteasome / Ubiquitin, Protein Trafficking, Proteolysis/Ubiquitin, RNA Processing, Signal Transduction, Spindle, Ubiquitin & Ubiquitin Like Modifiers

E3 SUMO-protein ligase which facilitates SUMO1 and SUMO2 conjugation by UBE2I. Involved in transport factor (Ran-GTP, karyopherin) -mediated protein import via the F-G repeat-containing domain which acts as a docking site for substrates. Binds single- stranded RNA (in vitro) . May bind DNA. Component of the nuclear export pathway. Specific docking site for the nuclear export factor exportin-1. Sumoylates PML at 'Lys-490' which is essential for the proper assembly of PML-NB. .

1. Beddow AL, Richards SA, Orem NR, Macara IG (Apr 1995) . The Ran/TC4 GTPase-binding domain: identification by expression cloning and characterization of a conserved sequence motif. Proceedings of the National Academy of Sciences of the United States of America. 92 (8) : 3328–32. 2. Yaseen NR, Blobel G (Sep 1999) . GTP hydrolysis links initiation and termination of nuclear import on the nucleoporin nup358. The Journal of Biological Chemistry. 274 (37) : 26493–502.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Nucleus . Nucleus membrane . Nucleus, nuclear pore complex . Detected in diffuse and discrete intranuclear foci (PubMed:11839768) . Cytoplasmic filaments (PubMed:7775481) . .