Anti-Vinculin/VCL Antibody Picoband® Fluoro488 Conjugated

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.

E.coli-derived human Vinculin recombinant protein (Position: E930-Q1134) . Human Vinculin shares 87.7% amino acid (aa) sequence identity with both mouse and rat Vinculin.

Actin filament (F-actin) -binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. .

1. Ezzell RM, Goldmann WH, Wang N, Parashurama N, Parasharama N, Ingber DE (1997) . Vinculin promotes cell spreading by mechanically coupling integrins to the cytoskeleton. Exp. Cell Res. 231 (1) : 14–26. 2. Goldmann WH, Ingber DE (2001) . Intact vinculin protein is Require for control of cell shape, cell mechanics, and rac-Dependent Lamellipodia Formation. Biochemical and Biophysical Research Communications. 290 (2) : 749–755. 3. Xu W, Baribault H, Adamson ED (1998) . vinculin knockout results in heart and brain defects during embryonic development. Development. 125 (2) : 327–337.

Cytoplasm, cytoskeleton . Cell junction, adherens junction . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction, focal adhesion . Cytoplasmic face of adhesion plaques. Recruitment to cell-cell junctions occurs in a myosin II- dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions (By similarity) . Colocalizes with LIMD1 in the focal adhesions. .