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Anti-Musashi 1/Msi1 Antibody Picoband®

Boster Bio Anti-Musashi 1/Msi1 Antibody Picoband® catalog # A05052-1. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

RNA-binding protein Musashi homolog 1 is a protein that in humans is encoded by the MSI1 gene. This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13.

Synonyms

RNA-binding protein Musashi homolog 1; Musashi-1; MSI1

Gene Name

MSI1

Gene ID

4440

UniProt

O43347

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Musashi 1/Msi1, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Tissue Specificity

Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines. .

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Cell Type Marker, Cytoplasmic, Intracellular, Neural Stem Cells, Neurogenesis, Neurology Process, Neuroscience, Nuclear, Signaling Pathways, Stem Cells

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'- GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'- [GA]U (1-3) AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similarity) . .

References & Citations

1. Good P, Yoda A, Sakakibara S, Yamamoto A, Imai T, Sawa H, Ikeuchi T, Tsuji S, Satoh H, Okano H (Dec 1998) . The human Musashi homolog 1 (MSI1) gene encoding the homologue of Musashi/Nrp-1, a neural RNA-binding protein putatively expressed in CNS stem cells and neural progenitor cells. Genomics. 52 (3) : 382–4.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

39125 MW

Observed Molecular Weight

39 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

RNA-binding protein Musashi homolog 1

Subcellular Location

Cytoplasm . Nucleus .

Protein Name

RNA-binding protein Musashi homolog 1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

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