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Anti-CD90/Thy1 Antibody Picoband®

Boster Bio Anti-CD90/Thy1 Antibody Picoband® catalog # A01818. Tested in IF, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

CD90 (Cluster of Differentiation 90) or Thy-1 is a 25–37 kDa heavily N-glycosylated, glycophosphatidylinositol (GPI) anchored conserved cell surface protein with a single V-like immunoglobulin domain, originally discovered as a thymocyte antigen. The CD90 gene is mapped to 11q23.3. Thy-1 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of Thy-1 lead to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to the first biochemical description and characterization of a vertebrate GPI anchor.

Synonyms

Thy-1 membrane glycoprotein; Thy-1 antigen; CD90; Thy1; Thy-1

Gene Name

Thy1

Gene ID

21838

UniProt

P01831

Host

Rabbit

Reactivity

Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived mouse CD90/Thy1 recombinant protein (Position: Q20-C131) . Mouse CD90/Thy1 shares 63.4% and 81.3% amino acid (aa) sequence identity with human and rat CD90/Thy1, respectively.

Clonality

Polyclonal

Tissue Specificity

Detected adjacent to nodes of Ranvier in juxtaparanodal zones in spinal cord nerve fibers, but also in paranodal regions in some myelinated spinal cord axons (at protein level) (PubMed:11086297) . Detected in the islet of Langerhans (PubMed:21483673) .

Applications

IF, IHC, WB

Field of Research

Neuroscience, Stem Cells, Synapse Marker, T Cells, T Lymphocytic Lineage

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

References & Citations

1. Abeysinghe, H. R., Cao, Q., Xu, J., Pollock, S., Veyberman, Y., Guckert, N. L., Keng, P., Wang, N.THY1 expression is associated with tumor suppression of human ovarian cancer.Cancer Genet. Cytogenet. 143: 125-132, 2003. 2. McKenzie, J. L., Fabre, J. W.Human Thy-1: unusual localization and possible functional significance in lymphoid tissues.J. Immun. 126: 843-850, 1981. 3. Morris, R.Thy-1 in developing nervous tissue.Dev. Neurosci. 7: 133-160, 1985.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

18080 MW

Observed Molecular Weight

22 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Thy-1 membrane glycoprotein

Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor.

Protein Name

Thy-1 membrane glycoprotein

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

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