Anti-TFPI Antibody Picoband® Fluoro647 Conjugated

TFPI (Tissue factor pathway inhibitor) also known as EPI, LACI, TFI and TFPT1 is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa) . While Xa is inhibited, the Xa-TFPI complex can subsequently also inhibit the FVIIa-tissue factor complex. TFPI contributes significantly to the inhibition of Xa in vivo, despite being present at concentrations of only 2.5 nM. The gene for TFPI is located on chromosome 2q31-q32.1, and has nine exons which span 70 kb. TFPI has a relative molecular mass of 34,000 to 40,000 depending on the degree of proteolysis of the C-terminal region.

E.coli-derived human TFPI recombinant protein (Position: N253-M304) . Human TFPI shares 51.1% and 53.2% amino acid (aa) sequence identity with mouse and rat TFPI, respectively.

Inhibits factor X (X (a) ) ly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.

1. Enjyoji, K.-I., Emi, M., Mukai, T., Imada, M., Leppert, M. L., Lalouel, J. M., Kato, H.Human tissue factor pathway inhibitor (TFPI) gene: complete genomic structure and localization on the genetic map of chromosome 2q.Genomics 17: 423-428, 1993. 2. Kleesiek, K., Schmidt, M., Gotting, C., Brinkmann, T., Prohaska, W.A first mutation in the human tissue factor pathway inhibitor gene encoding (P151L) TFPI. (Letter) Blood 92: 3976-3977, 1998. 3. Lupu, C., Zhu, H., Popescu, N. I., Wren, J. D., Lupu, F.Novel protein ADTRP regulates TFPI expression and function in human endothelial cells in normal conditions and in response to androgen.Blood 118: 4463-4471, 2011.