Anti-SPARC Antibody Picoband® Fluoro647 Conjugated

SPARC, secreted protein acidic and rich in cysteine, also known as Osteonectin is a protein that in humans is encoded by the SPARC gene. The human SPARC gene is 26.5 kb long, and contains 10 exons and 9 introns and is located on chromosome 5q31-q33. SPARC is an acidic, cysteine-rich glycoprotein consisting of a single polypeptide chain that can be broken into 4 domains: 1) an Ca++ binding domains near the glutamic acidic-rich region at the amino terminus (domain I), 2) a cysteine- rich (domain II), 3) a hydrophilic region (domain III) and 4) an EF hand motif at the carboxy terminus region (domain IV) . Furthermore, SPARC is a glycoprotein in the bone that binds sodium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. SPARC also shows affinity for collagen in addition to bone mineral calcium. A correlation between SPARC over expression and ampullary cancers and chronic pancreatitis has been found.

E.coli-derived human SPARC recombinant protein (Position: E107-I303) . Human SPARC shares 97% amino acid (aa) sequence identity with both mouse and rat SPARC.

Angiogenesis, Cancer, Cardiovascular, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Cytoskeleton/ECM, Developmental Biology, Extracellular Matrix, Lineage Markers, Lineage Specification, Mesenchymal Stem Cells, Protein Phosphorylation, Receptor Tyrosine Kinases, Signal Transduction, Stem Cells, Structures, Tyrosine Kinases

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca (2+) with a low affinity and an EF-hand loop that binds a Ca (2+) ion with a high affinity.

1. Gilmour, D. T., Lyon, G. J., Carlton, M. B. L., Sanes, J. R., Cunningham, J. M., Anderson, J. R., Hogan, B. L. M., Evans, M. J., Colledge, W. H.Mice deficient for the secreted glycoprotein SPARC/osteonectin/BM40 develop normally but show severe age-onset cataract formation and disruption of the lens.EMBO J. 17: 1860-1870, 1998. 2. Goldblum, S. E., Ding, X., Funk, S. E., Sage, E. H.SPARC (secreted protein acidic and rich in cysteine) regulates endothelial cell shape and barrier function.Proc. Nat. Acad. Sci. 91: 3448-3452, 1994. 3. Minn, A. J., Gupta, G. P., Siegel, P. M., Bos, P. D., Shu, W., Giri, D. D., Viale, A., Olshen, A. B., Gerald, W. L., Massague, J.Genes that mediate breast cancer metastasis to lung.Nature 436: 518-524, 2005.