Anti-CPI17 alpha/PPP1R14A Antibody Picoband® Fluoro488 Conjugated
Product Specifications
Background
Protein phosphatase 1 regulatory subunit 14A, also known as CPI-17, is a protein that in humans is encoded by the PPP1R14A gene. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Synonyms
Protein phosphatase 1 regulatory subunit 14A;17 kDa PKC-potentiated inhibitory protein of PP1; Protein kinase C-potentiated inhibitor protein of 17 kDa; CPI-17; PPP1R14A; CPI17, PPP1INL
Gene Name
PPP1R14A
Gene ID
94274
UniProt
Q96A00
Host
Rabbit
Reactivity
Human, Mouse, Rat
Cross Reactivity
No cross-reactivity with other proteins
Immunogen
E.coli-derived human CPI17 alpha recombinant protein (Position: L30-Q126) . Human CPI17 alpha shares 83.5% and 84.5% amino acid (aa) sequence identity with mouse and rat CPI17 alpha, respectively.
Clonality
Polyclonal
Tissue Specificity
Isoform 1 is detected in aorta and testis. Isoform 2 is detected in aorta. .
Applications
Flow Cytometry
Field of Research
Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction
Purification
Immunogen affinity purified.
Form
Liquid
Function
Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.
References & Citations
1. Eto M, Ohmori T, Suzuki M, et al. (1996) . A novel protein phosphatase-1 inhibitory protein potentiated by protein kinase C. Isolation from porcine aorta media and characterization.. J. Biochem. 118 (6) : 1104–7. 2. Yamawaki K, Ito M, Machida H, Moriki N, Okamoto R, Isaka N, Shimpo H, Kohda A, Okumura K, Hartshorne DJ, Nakano T (Jul 2001) . Identification of human CPI-17, an inhibitory phosphoprotein for myosin phosphatase. Biochem Biophys Res Commun. 285 (4) : 1040–5.
Storage Conditions
At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Calculated Molecular Weight
16693 MW
Specificity
No cross reactivity with other proteins.
Applications Notes
6
Gene Name Synonym
Protein phosphatase 1 regulatory subunit 14A
Subcellular Location
Cytoplasm .
Protein Name
Protein phosphatase 1 regulatory subunit 14A
Isotype
Rabbit IgG
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
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