Anti-Protein Kinase A regulatory subunit I alpha/PRKAR1A Antibody Picoband®

CAMP-dependent protein kinase type I-alpha regulatory subunit is an enzyme that in humans is encoded by the PRKAR1A gene. This protein encoded by this gene was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC) . This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40) . Several alternatively spliced transcript variants encoding two different isoforms have been observed.

CAMP-dependent protein kinase type I-alpha regulatory subunit; Tissue-specific extinguisher 1; TSE1; cAMP-dependent protein kinase type I-alpha regulatory subunit, N-terminally processed; PRKAR1A; PKR1, PRKAR1, TSE1

E.coli-derived human PRKAR1A recombinant protein (Position: E2-E81) . Human PRKAR1A shares 89.9% and 92.4% amino acid (aa) sequence identity with mouse and rat PRKAR1A, respectively.

Four types of regulatory chains are found: I- alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

Cancer, Cell Cycle, DNA/RNA, Epigenetics and Nuclear Signaling, Kinases/Phosphatases, Nuclear Import/Export, Protein Trafficking, RNA Processing, Signal Transduction

1. Entrez Gene: PRKAR1A protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) . 2. Scambler P, Oyen O, Wainwright B, Farrall M, Law HY, Estivill X, Sandberg M, Williamson R, Jahnsen T (December 1987) . Exclusion of catalytic and regulatory subunits of cAMP-dependent protein kinase as candidate genes for the defect causing cystic fibrosis. Am J Hum Genet. 41 (5) : 925–32.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.