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Anti-CKAP4 Antibody Picoband®

Boster Bio Anti-CKAP4 Antibody Picoband® catalog # A02154-1. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Cytoskeleton-associated protein 4, also known as CLIMP63 and p63, is a protein that in humans is encoded by the CKAP4 gene. It is mapped to 12q23.3. CKAP4 is found on the plasma membrane of select cells such as vascular smooth muscle and Type II Greater alveolar lung cells. It has a bimodal distribution. First, CKAP4 is found on the plasma membrane and serves as a receptor for SP-A/surfactant protein-A (in lung) and tPA (in vessels) . Second, it is embedded in the membrane of a compartment that links the ER with the Golgi apparatus. This localization is dependent on its ability to form homooligomers, and its presence serves to anchor microtubules and the formation of tubular ER.

Synonyms

Cytoskeleton-associated protein 4;63-kDa cytoskeleton-linking membrane protein; Climp-63; p63; Ckap4

Gene Name

Ckap4

Gene ID

216197

UniProt

Q8BMK4

Host

Rabbit

Reactivity

Mouse

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of mouse CKAP4, different from the related human sequence by one amino acid.

Clonality

Polyclonal

Tissue Specificity

High in intestine and in peripheral blood mononuclear cells; low in lung, kidney, thymus, uterus, vasculature and brain. Not found in liver, heart, retina oe skeletal muscle.

Applications

WB

Field of Research

Cancer, Tumor Biomarkers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

High-affinity epithelial cell surface receptor for APF. .

References & Citations

1. Entrez Gene: CKAP4 cytoskeleton-associated protein 4. 2. Schweizer A, Rohrer J, Jeno P, DeMaio A, Buchman TG, Hauri HP (Jul 1993) . A reversibly palmitoylated resident protein (p63) of an ER-Golgi intermediate compartment is related to a circulatory shock resuscitation protein. J Cell Sci. 104 (3) : 685–94.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

63692 MW

Observed Molecular Weight

66 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Cytoskeleton-associated protein 4

Subcellular Location

Endoplasmic reticulum membrane; Single-pass type II membrane protein. Cell membrane; Single-pass type II membrane protein. Cytoplasm, cytoskeleton. Cytoplasm, perinuclear region. Translocates to the perinuclear region upon APF- stimulation. .

Protein Name

Cytoskeleton-associated protein 4

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

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