What sizes are available for Anti-Iba1/AIF1 Antibody Picoband® Fluoro488 Conjugated?

Anti-Iba1/AIF1 Antibody Picoband® Fluoro488 Conjugated is available in the following size(s): 100 µg/Vial.

How many freeze-thaw cycles can this product tolerate?

We recommend aliquoting the product upon receipt to avoid repeated freeze-thaw cycles, which can reduce activity and stability. This is your responsibility once the product has been delivered.

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

What temperature does this product ship at?

Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.

Anti-Iba1/AIF1 Antibody Picoband® Fluoro488 Conjugated

Q: What is the catalog number for Anti-Iba1/AIF1 Antibody Picoband® Fluoro488 Conjugated?

The catalog number for Anti-Iba1/AIF1 Antibody Picoband® Fluoro488 Conjugated is A01394-Fluoro488.

Q: Is Anti-Iba1/AIF1 Antibody Picoband® Fluoro488 Conjugated in stock?

Anti-Iba1/AIF1 Antibody Picoband® Fluoro488 Conjugated is currently made to order / not held in immediate stock. Contact us for lead time.

Product Specifications

Background

Allograft inflammatory factor 1 (AIF-1), also known as ionized calcium-binding adapter molecule 1 (IBA1), is a protein that in humans is encoded by the AIF1 gene. This gene encodes a protein that binds actin and calcium. And this gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain.

Synonyms

Allograft inflammatory factor 1; AIF-1; Ionized calcium-binding adapter molecule 1; Protein G1; AIF1; G1, IBA1

Gene Name

AIF1

Gene ID

199

UniProt

P55008

Host

Rabbit

Reactivity

Human, Mouse, Rat, Pig

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Iba1, different from the related mouse sequence by seven amino acids, and from the related rat sequence by six amino acids.

Clonality

Polyclonal

Tissue Specificity

Detected in T-lymphocytes and peripheral blood mononuclear cells. .

Applications

Flow Cytometry

Field of Research

Actin Binding Proteins, Actin, etc., Cytoskeleton, Cytoskeleton/ECM, Microfilaments, Signal Transduction

Purification

Immunogen affinity purified.

Form

Liquid

Function

Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T- lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. .

References & Citations

1. Entrez Gene: AIF1 allograft inflammatory factor 1. 2. Utans U, Arceci RJ, Yamashita Y, Russell ME (June 1995) . Cloning and characterization of allograft inflammatory factor-1: a novel macrophage factor identified in rat cardiac allografts with chronic rejection. J. Clin. Invest. 95 (6) : 2954–62. 3. Tian Y, Jain S, Kelemen SE, Autieri MV (February 2009) . AIF-1 expression regulates endothelial cell activation, signal transduction, and vasculogenesis. Am. J. Physiol., Cell Physiol. 296 (2) : C256–66.

Storage Conditions

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Calculated Molecular Weight

16703 MW

Specificity

No cross reactivity with other proteins.

Applications Notes

Gene Name Synonym

Allograft inflammatory factor 1

Subcellular Location

Cytoplasm, cytoskeleton. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.