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Anti-Beta Tubulin/TUBB Antibody Picoband®

Boster Bio Anti-Beta Tubulin/TUBB Antibody Picoband® catalog # A01857-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat, Chicken. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Tubulin beta chain is a protein that in humans is encoded by the TUBB gene. This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13.

Synonyms

Tubulin beta chain; Tubulin beta-5 chain; TUBB; TUBB5; OK/SW-cl.56

Gene Name

TUBB

Gene ID

203068

UniProt

P07437

Host

Rabbit

Reactivity

Chicken, Human, Monkey, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Beta Tubulin, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Tissue Specificity

Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

Applications

IF, IHC, ICC, WB, Flow Cytometry

Field of Research

Cytoskeleton, Cytoskeleton/ECM, Microtubules, Signal Transduction, Subcellular Markers, Tags & Cell Markers, Tubulin

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

References & Citations

1. Entrez Gene: TUBB tubulin, beta. 2. Volz A, Weiss E, Trowsdale J, Ziegler A (Jan 1994) . Presence of an expressed beta-tubulin gene (TUBB) in the HLA class I region may provide the genetic basis for HLA-linked microtubule dysfunction. Human Genetics. 93 (1) : 42–6.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

50433 MW

Observed Molecular Weight

50 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Tubulin beta class I

Subcellular Location

Cytoskeleton

Protein Name

Tubulin beta chain

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

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