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Anti-Vesicular Acetylcholine Transporter/SLC18A3 Antibody Picoband®

Selected Variant

A04891

Size: 100 µg/Vial

Boster Bio Anti-Vesicular Acetylcholine Transporter/SLC18A3 Antibody Picoband® catalog # A04891. Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

The Vesicular acetylcholine transporter (VAChT), also known as SLC18A3, is a neurotransmitter transporter which is responsible for loadingacetylcholine (ACh) into secretory organelles in neurons making acetylcholine available for secretion. It is encoded by Solute carrier family 18, member 3 (SLC18A3) gene. This gene is a member of the vesicular amine transporter family. The encoded transmembrane protein transports acetylcholine into secretory vesicles for release into the extracellular space. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene.

Synonyms

Vesicular acetylcholine transporter; VAChT; Solute carrier family 18 member 3; SLC18A3; VACHT

Gene Name

SLC18A3

Gene ID

6572

UniProt

Q16572

Host

Rabbit

Reactivity

Human, Mouse

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human SLC18A3, different from the related mouse and rat sequences by five amino acids.

Clonality

Polyclonal

Tissue Specificity

Peripheral and central cholinergic nervous systems. .

Applications

WB

Field of Research

Neuroscience, Neurotransmitter

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Involved in acetylcholine transport into synaptic vesicles. .

References & Citations

1. Erickson JD, Varoqui H (Dec 2000) . Molecular analysis of vesicular amine transporter function and targeting to secretory organelles. FASEB Journal. 14 (15) : 2450–8. 2. Weihe E, Tao-Cheng JH, Schäfer MK, Erickson JD, Eiden LE (Apr 1996) . Visualization of the vesicular acetylcholine transporter in cholinergic nerve terminals and its targeting to a specific population of small synaptic vesicles. Proceedings of the National Academy of Sciences of the United States of America. 93 (8) : 3547–52.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

56903 MW

Observed Molecular Weight

55 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Vesicular acetylcholine transporter

Subcellular Location

Membrane ; Multi-pass membrane protein .

Protein Name

Vesicular acetylcholine transporter

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Price

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