Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

Anti-NEDD8 Antibody Picoband® Fluoro647 Conjugated

Product Specifications

Background

NEDD8 is a protein that in humans is encoded by the NEDD8 gene. Human NEDD8 shares 60% amino acid sequence identity to ubiquitin. The most substrates of NEDD8 modification are the Cullin subunits of Cullin-based E3 ubiquitin ligases, which are active only when neddylated. Their NEDDylation is critical for the recruitment of E2 to the ligase complex, thus facilitating ubiquitin conjugation. NEDD8 modification has therefore been implicated in cell cycle progression and cytoskeletal regulation.

Synonyms

NEDD8; Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; NEDD-8; Ubiquitin-like protein Nedd8; NEDD8

Gene Name

NEDD8

Gene ID

4738

UniProt

Q15843

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human NEDD8, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Tissue Specificity

Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes. .

Applications

Flow Cytometry

Field of Research

Cell Biology, Proteasome / Ubiquitin, Proteolysis/Ubiquitin, Ub-Like Proteins

Purification

Immunogen affinity purified.

Form

Liquid

Function

Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C- APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. .

References & Citations

1. Bohnsack, R. N., Haas, A. L. Conservation in the mechanism of Nedd8 activation by the human AppBp1-Uba3 heterodimer. J. Biol. Chem. 278: 26823-26830, 2003. 2. Cope, G. A., Suh, G. S. B., Aravind, L., Schwarz, S. E., Zipursky, S. L., Koonin, E. V., Deshaies, R. J. Role of predicted metalloprotease motif of Jab1/Csn5 in cleavage of Nedd8 from Cul1. Science 298: 608-611, 2002. 3. Cui, J., Yao, Q., Li, S., Ding, X., Lu, Q., Mao, H., Liu, L., Zheng, N., Chen, S., Shao, F. Glutamine deamidation and dysfunction of ubiquitin/NEDD8 induced by a bacterial effector family. Science 329: 1215-1218, 2010.

Storage Conditions

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Calculated Molecular Weight

9072 MW

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

NEDD8

Subcellular Location

Nucleus . Mainly nuclear.

Protein Name

NEDD8

Isotype

Rabbit IgG

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

pCMV-SPORT6-Aldh1l1 Plasmid
PVT56990 2 µg

pCMV-SPORT6-Aldh1l1 Plasmid

Ask
View Details
BTG3 Polyclonal Antibody
RD89511A-01 60 μL

BTG3 Polyclonal Antibody

Ask
View Details
BTG3 Polyclonal Antibody
RD89511A-02 120 μL

BTG3 Polyclonal Antibody

Ask
View Details
BTG3 Polyclonal Antibody
RD89511A-03 200 µL

BTG3 Polyclonal Antibody

Ask
View Details
Recombinant Scheffersomyces stipitis Translation machinery-associated protein 22 (TMA22)
MBS1225552-01 0.05 mg (E-Coli)

Recombinant Scheffersomyces stipitis Translation machinery-associated protein 22 (TMA22)

Ask
View Details
Recombinant Scheffersomyces stipitis Translation machinery-associated protein 22 (TMA22)
MBS1225552-02 0.05 mg (Yeast)

Recombinant Scheffersomyces stipitis Translation machinery-associated protein 22 (TMA22)

Ask
View Details