Anti-KCNQ1 Antibody Picoband®

Kv7.1 (KvLQT1) is a potassium channel protein whose primary subunit in humans is encoded by the KCNQ1 gene. This protein can form heteromultimers with two other potassium channel proteins, KCNE1 and KCNE3. Mutations in this gene are associated with hereditary long QT syndrome 1 (also known as Romano-Ward syndrome), Jervell and Lange-Nielsen syndrome, and familial atrial fibrillation. This gene exhibits tissue-specific imprinting, with preferential expression from the maternal allele in some tissues, and biallelic expression in others. And this gene is located in a region of chromosome 11 amongst other imprinted genes that are associated with Beckwith-Wiedemann syndrome (BWS), and itself has been shown to be disrupted by chromosomal rearrangements in patients with BWS. Alternatively spliced transcript variants have been found for this gene.

Potassium voltage-gated channel subfamily KQT member 1 ; IKs producing slow voltage-gated potassium channel subunit alpha KvLQT1 ; KQT-like 1 ; Voltage-gated potassium channel subunit Kv7.1 ; KCNQ1 ; KCNA8 , KCNA9 , KVLQT1

A synthetic peptide corresponding to a sequence in the middle region of human KCNQ1, different from the related mouse sequence by two amino acids, and from the related rat sequence by one amino acid.

Abundantly expressed in heart, pancreas, prostate, kidney, small intestine and peripheral blood leukocytes. Less abundant in placenta, lung, spleen, colon, thymus, testis and ovaries.

Potassium channel that plays an important role in a number of tissues, including heart, inner ear, stomach and colon (By similarity) (PubMed:10646604) . Associates with KCNE beta subunits that modulates current kinetics (By similarity) (PubMed:9312006, PubMed:9108097, PubMed:8900283, PubMed:10646604, PubMed:11101505, PubMed:19687231) . Induces a voltage-dependent by rapidly activating and slowly deactivating potassium-selective outward current (By similarity) (PubMed:9312006, PubMed:9108097, PubMed:8900283, PubMed:10646604, PubMed:11101505) . Promotes also a delayed voltage activated potassium current showing outward rectification characteristic (By similarity) . During beta- adrenergic receptor stimulation participates in cardiac repolarization by associating with KCNE1 to form the I (Ks) cardiac potassium current that increases the amplitude and slows down the activation kinetics of outward potassium current I (Ks) (By similarity) (PubMed:9312006, PubMed:9108097, PubMed:8900283, PubMed:10646604, PubMed:11101505) . Muscarinic agonist oxotremorine-M strongly suppresses KCNQ1/KCNE1 current (PubMed:10713961) . When associated with KCNE3, forms the potassium channel that is important for cyclic AMP-stimulated intestinal secretion of chloride ions (PubMed:10646604) . This interaction with KCNE3 is reduced by 17beta-estradiol, resulting in the reduction of currents (By similarity) . During conditions of increased substrate load, maintains the driving force for proximal tubular and intestinal sodium ions absorption, gastric acid secretion, and cAMP-induced jejunal chloride ions secretion (By similarity) . Allows the provision of potassium ions to the luminal membrane of the secretory canaliculus in the resting state as well as during stimulated acid secretion (By similarity) . When associated with KCNE2, forms a heterooligomer complex leading to currents with an apparently instantaneous activation, a rapid deactivation process and a linear current-voltage relationship and decreases the amplitude of the outward current (PubMed:11101505) . When associated with KCNE4, inhibits voltage-gated potassium channel activity (PubMed:19687231) . When associated with KCNE5, this complex only conducts current upon strong and continued depolarization (PubMed:12324418) . Also forms a heterotetramer with KCNQ5; has a voltage-gated potassium channel activity (PubMed:24855057) . Binds with phosphatidylinositol 4,5- bisphosphate (PubMed:25037568) . .

1. Entrez Gene: KCNQ1 potassium voltage-gated channel, KQT-like subfamily, member 1. 2. Jespersen T, Grunnet M, Olesen SP (2005) . The KCNQ1 potassium channel: from gene to physiological function. Physiology (Bethesda) . 20 (6) : 408–16. 3. Torekov SS, Iepsen E, Christiansen M, Linneberg A, Pedersen O, Holst JJ, Kanters JK, Hansen T (2014) . KCNQ1 Long QT syndrome patients have hyperinsulinemia and symptomatic hypoglycemia.) . Diabetes. 63 (4) : 1315–25.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Cell membrane ; Multi- pass membrane protein . Cytoplasmic vesicle membrane . Early endosome . Membrane raft . Endoplasmic reticulum . Basolateral cell membrane . Colocalized with KCNE3 at the plasma membrane (PubMed:10646604) . Upon 17beta-oestradiol treatment, colocalizes with RAB5A at early endosome (PubMed:23529131) . Heterotetramer with KCNQ5 is highly retained at the endoplasmic reticulum and is localized outside of lipid raft microdomains (PubMed:24855057) . During the early stages of epithelial cell polarization induced by the calcium switch it removed from plasma membrane to the endoplasmic reticulum where it retained and it is redistributed to the basolateral cell surface in a PI3K-dependent manner at a later stage (PubMed:21228319) . .