Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

Anti-E2F3 Antibody Picoband®

Boster Bio Anti-E2F3 Antibody Picoband® catalog # A03068. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Transcription factor E2F3, also known as KIAA0075, is a protein that in humans is encoded by the E2F3 gene. The protein encoded by this gene is a member of the E2F family of transcription factors. By fluorescence in situ hybridization, E2F3 gene is mapped to 6q22.3. The induction of specific E2F activities is an essential component in the MYC pathways that control cell proliferation and cell fate decisions. This gene encodes a member of a small family of transcription factors that function through binding of DP interaction partner proteins. The encoded protein recognizes a specific sequence motif in DNA and interacts ly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle.

Synonyms

Transcription factor E2F3; E2F-3; E2F3; KIAA0075

Gene Name

E2F3

Gene ID

1871

UniProt

O00716

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human E2F3, identical to the related mouse sequence.

Clonality

Polyclonal

Tissue Specificity

Expressed in skeletal muscle, kidney, heart, placenta, liver, thymus, lung, and ovary, with little or no expression in other tissues.

Applications

WB

Field of Research

Domain Families, Epigenetics and Nuclear Signaling, Forkhead Box, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner. Inhibits adipogenesis, probably through the repression of CEBPA binding to its target gene promoters (By similarity) . .

References & Citations

1. Chen, D., Pacal, M., Wenzel, P., Knoepfler, P. S., Leone, G., Bremner, R. Division and apoptosis of E2f-deficient retinal progenitors. Nature 462: 925-929, 2009. 2. Chong, J.-L., Wenzel, P. L., Saenz-Robles, M. T., Nair, V., Ferrey, A., Hagan, J. P., Gomez, Y. M., Sharma, N., Chen, H.-Z., Ouseph, M., Wang, S.-H., Trikha, P., and 10 others. E2f1-3 switch from activators in progenitor cells to repressors in differentiating cells. Nature 462: 930-934, 2009.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

49162 MW

Observed Molecular Weight

49 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Transcription factor E2F3

Subcellular Location

Nucleus.

Protein Name

Transcription factor E2F3

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

Iron (metal) powder 99.5% Electrolytic (250-300 mesh)
01471 05000 5 Kg

Iron (metal) powder 99.5% Electrolytic (250-300 mesh)

Ask
View Details
Human MTNR1B Pre-designed siRNA Set A
SI010006A 1 Each

Human MTNR1B Pre-designed siRNA Set A

Ask
View Details
Rimkla (NM_177572) Mouse Tagged ORF Clone Lentiviral Particle
MR205935L3V 200 µL

Rimkla (NM_177572) Mouse Tagged ORF Clone Lentiviral Particle

Ask
View Details
Mouse CSNK2A1 shRNA Plasmid
abx969856-01 150 µg

Mouse CSNK2A1 shRNA Plasmid

Ask
View Details
Mouse CSNK2A1 shRNA Plasmid
abx969856-02 300 µg

Mouse CSNK2A1 shRNA Plasmid

Ask
View Details
Mouse Cog6 (NM_026225) AAV Particle
MR209346A1V 250 µL

Mouse Cog6 (NM_026225) AAV Particle

Ask
View Details