Anti-Angiogenin/ANG Antibody Picoband® Fluoro488 Conjugated

Angiogenin (Ang), also known as ribonuclease 5, is a small 123 amino acid protein that in humans is encoded by the ANG gene. The protein encoded by this gene is an exceedingly potent mediator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. In addition, the mature peptide has antimicrobial activity against some bacteria and fungi, including S. pneumoniae and C. albicans. Alternative splicing results in two transcript variants encoding the same protein. This gene and the gene that encodes ribonuclease, RNase A family, 4 share promoters and 5' exons. Each gene splices to a unique downstream exon that contains its complete coding region.

E. coli-derived human Angiogenin/ANG recombinant protein (Position: Q25-P147) . Human Angiogenin/ANG shares 77.5% amino acid (aa) sequence identity with mouse Angiogenin/ANG.

Actin Binding Proteins, Actin, etc., Angiogenesis, Cancer, Cancer Metabolism, Cardiovascular, Cytoskeleton, Cytoskeleton/ECM, DNA/RNA, Epigenetics and Nuclear Signaling, Hypoxia, Invasion/Microenvironment, Metabolism, Metabolism Processes, Microfilaments, Neurodegenerative Disease, Neurology Process, Neuroscience, Pathways and Processes, Response To Hypoxia, RNA Processing, Signal Transduction, Translation

Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs) . Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo. .

1. Gao X, Xu Z (2008) . Mechanisms of action of angiogenin. Acta Biochimica et Biophysica Sinica. 40 (7) : 619–624. 2. Tello-Montoliu A; Patel J.V.; Lip G.Y.H. (2006) . Angiogenin: a review of the pathophysiology and potential clinical applications. Journal of Thrombosis and Haemostasis. 4 (9) : 1864–74. 3. Tsuji T, Sun Y, Kishimoto K, Olson K, Luo S, Hirukawa S, Hu G (2005) . Angiogenin is translocated to the nucleus of HeLa cells and is involved in ribosomal RNA transcription and cell proliferation. Cancer Research (65) : 1352.

Nucleus . Secreted, extracellular space, extracellular matrix, basement membrane. Nucleus, nucleolus. Rapidly endocytosed by target cells and translocated to the nucleus where it accumulates in the nucleolus and binds to DNA.