Anti-Werner's syndrome helicase WRN/WRN Antibody Picoband® Fluoro647 Conjugated

Werner syndrome ATP-dependent helicase, also known as DNA helicase, RecQ-like type 3, is an enzyme that in humans is encoded by the WRN gene. This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by premature aging.

Werner syndrome ATP-dependent helicase;3.6.4.12 ; DNA helicase, RecQ-like type 3; RecQ3; Exonuclease WRN;3.1.-.-; RecQ protein-like 2; WRN; RECQ3, RECQL2

Multifunctional enzyme that has both magnesium and ATP- dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double- stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity) . Plays a role in double-strand break repair after gamma-irradiation. .

1. Ding SL, Shen CY (2008) . Model of human aging: recent findings on Werner's and Hutchinson-Gilford progeria syndromes. Clin Interv Aging3 (3) : 431–44. 2. Rossi ML, Ghosh AK, Bohr VA (2010) . Roles of Werner syndrome protein in protection of genome integrity. DNA Repair (Amst.) 9 (3) : 331–44.

Nucleus, nucleolus . Nucleus . Nucleus, nucleoplasm . Gamma- irradiation leads to its translocation from nucleoli to nucleoplasm and PML regulates the irradiation-induced WRN relocation. .