Anti-NR2F6 Antibody Picoband®

Nuclear receptor subfamily 2 group F member 6 is a protein that in humans is encoded by the NR2F6 gene. It is a member of the nuclear receptor family of intracellular transcription factors. It has been shown to function as a coregulator of other nuclear receptors. NR2F6 impairs the formation of mature red blood cells in animals that over-express NR2F6 in their bone marrow. Mice that over expression of NR2F6 in their bone marrow cells have a block at the pro-erythroblast stage of blood cell development both in the bone marrow and in the spleen of animals that have excessive expression of NR2F6. So, when inhibition of differentiation of stem cell is desired, inhibition of differentiation is achieved through induction of increased NR2F6 activity. In situations where differentiation of stem cells into a cell of increased maturity is desired, inhibition of NR2F6 activity must be performed.

E. coli-derived human NR2F6 recombinant protein (Position: M270-Q404) . Human NR2F6 shares 99.3% amino acid (aa) sequence identity with both mouse and rat NR2F6.

Transcription factor predominantly involved in transcriptional repression. Binds to promoter/enhancer response elements that contain the imperfect 5'-AGGTCA-3' or inverted repeats with various spacings which are also recognized by other nuclear hormone receptors. Involved in modulation of hormonal responses. Represses transcriptional activity of the lutropin-choriogonadotropic hormone receptor/LHCGR gene, the renin/REN gene and the oxytocin-neurophysin/OXT gene. Represses the triiodothyronine-dependent and -independent transcriptional activity of the thyroid hormone receptor gene in a cell type- specific manner. The corepressing function towards thyroid hormone receptor beta/THRB involves at least in part the inhibition of THRB binding to triiodothyronine response elements (TREs) by NR2F6. Inhibits NFATC transcription factor DNA binding and subsequently its transcriptional activity. Acts as transcriptional repressor of IL-17 expression in Th-17 differentiated CD4 (+) T cells and may be involved in induction and/or maintenance of peripheral immunological tolerance and autoimmunity. Involved in development of forebrain circadian clock; is required early in the development of the locus coeruleus (LC) . .

1. Ichim, C.V. and Ichim, T., (2015) . Modulation of Hematopoietic Stem Cell Differentiation. U.S. Patent 20,150,299,712. 2. Warnecke M, Oster H, Revelli JP, Alvarez-Bolado G, Eichele G (March 2005) . Abnormal development of the locus coeruleus in Ear2 (Nr2f6) -deficient mice impairs the functionality of the forebrain clock and affects nociception. Genes & Development 19 (5) : 614–25.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.