Anti-TXNL2/GLRX3 Antibody Picoband®

Glutaredoxin-3 is a protein that in humans is encoded by the GLRX3 gene. This gene encodes a member of the glutaredoxin family. Glutaredoxins are oxidoreductase enzymes that reduce a variety of substrates using glutathione as a cofactor. The encoded protein binds to and modulates the function of protein kinase C theta. The encoded protein may also inhibit apoptosis and play a role in cellular growth, and the expression of this gene may be a marker for cancer. Pseudogenes of this gene are located on the short arm of chromosomes 6 and 9. Alternatively spliced transcript variants have been observed for this gene.

Glutaredoxin-3; PKC-interacting cousin of thioredoxin; PICOT; PKC-theta-interacting protein; PKCq-interacting protein; Thioredoxin-like protein 2; GLRX3; PICOT, TXNL2; HUSSY-22

E. coli-derived human TXNL2 recombinant protein (Position: L89-S177) . Human TXNL2 shares 93.3% and 94.4% amino acid (aa) sequence identity with mouse and rat TXNL2, respectively.

Expressed in heart, spleen, testis and, to a lower extent, in thymus and peripheral blood leukocytes. Weakly expressed in lung, placenta, colon and small intestine.

Critical negative regulator of cardiac hypertrophy and a positive inotropic regulator (By similarity) . Crucial regulator of cellular iron homeostasis and hemoglobin maturation. May play a role in regulating the function of the thioredoxin system. Does not posses any thyoredoxin activity since it lacks the conserved motif that is essential for catalytic activity. .

1. Stanchi F, Bertocco E, Toppo S, Dioguardi R, Simionati B, Cannata N, Zimbello R, Lanfranchi G, Valle G (Jan 2001) . Characterization of 16 novel human genes showing high similarity to yeast sequences. Yeast 18 (1) : 69–80. 2. Witte S, Villalba M, Bi K, Liu Y, Isakov N, Altman A (Feb 2000) . Inhibition of the c-Jun N-terminal kinase/AP-1 and NF-kappaB pathways by PICOT, a novel protein kinase C-interacting protein with a thioredoxin homology domain. J Biol Chem 275 (3) : 1902–9.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Cytoplasm, cell cortex . Cytoplasm, myofibril, sarcomere, Z line . Under the plasma membrane. After PMA stimulation, GLRX3 and PRKCQ/PKC-theta translocate to a more extended submembrane area. In the Z line, found associated with CSRP3 (By similarity) . .